Duck lymphocytes. VIII. T-lymphoblastoid cell lines from reticuloendotheliosis virus-induced tumours

Avian Pathology : Journal of the W.V.P.A
S W ChanD A Higgins

Abstract

The T strain of reticuloendotheliosis virus (REV-T) obtained, along with the helper chicken syncytia virus (CSV), from the CSO4 cell line was highly oncogenic and rapidly fatal in ducks. Tumours were mainly seen in spleen, but neoplastic cells were observed microscopically in many organs. In vitro REV transformation of duck lymphocytes failed to yield stable cell lines, so cells from organs (blood, bone marrow, spleen, lymph node, bursa of Fabricius) of infected birds were used to establish cell lines. Some of these cell lines have been cloned. The success rates of establishment and cloning were increased if cells were cultured in a range of media containing different supplements; however, medium containing 5% foetal calf serum (FCS) and 5% duck serum was generally most efficacious for initial establishment, while spent medium from the parental line supplemented with a further 20% FCS gave best results for cloning. Cloned cell lines had the morphology of lymphoblastoid cells, with irregular nuclei and diffuse chromatin. Analysis of mRNA extracted from these cell lines showed that the uncloned lines were strongly expressing the β chain of the T cell antigen receptor (TCR) and weakly expressing immunoglobulin (Ig) polypeptides [λ...Continue Reading

References

Jul 1, 1977·Journal of the National Cancer Institute·E C HahnA J Kenyon
Jan 15, 1978·International Journal of Cancer. Journal International Du Cancer·B W CalnekK A Schat
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·L W TjoelkerC B Thompson
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·L ChenJ M Bishop
Jan 21, 1988·Journal of Immunological Methods·D A Higgins, C S Teoh
Feb 12, 1986·Journal of Immunological Methods·D A Higgins, S H Chung
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Mar 1, 1980·Journal of Virology·I S Chen, H M Temin
Jan 1, 1995·Veterinary Immunology and Immunopathology·D A Higgins, W K Ko
Nov 1, 1994·Developmental and Comparative Immunology·K E MagorG W Warr
Jul 1, 1994·Poultry Science·C H ChenM D Cooper
Jun 1, 1996·Veterinary Immunology and Immunopathology·E M BertramI Kotlarski
Jun 1, 1993·Avian Pathology : Journal of the W.V.P.A·D A Higgins, G W Warr
Jan 1, 1987·Avian Pathology : Journal of the W.V.P.A·K Nazerian

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Citations

Mar 16, 2000·Developmental and Comparative Immunology·D A HigginsZ V Kounev
Mar 16, 2000·Developmental and Comparative Immunology·R L CromieJ L Stanford
Mar 16, 2000·Developmental and Comparative Immunology·A R Jilbert, I Kotlarski

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