Duct system of the rabbit lacrimal gland: structural characteristics and role in lacrimal secretion.

Investigative Ophthalmology & Visual Science
Chuanqing DingYanru Wang

Abstract

To develop a nomenclature for the lacrimal duct system in the rabbit, based on the anatomic and structural characteristics of each duct segment, and to provide RT-PCR and immunofluorescence data to support the notion that the duct system plays important roles in lacrimal function. Paraffin-embedded lacrimal glands (LGs) were stained with hematoxylin and eosin (H&E) and evaluated with a stereomicroscope. Cryosections of LG were stained with cresyl violet, and acinar cells and ductal epithelial cells were isolated from each duct segment by laser capture microdissection (LCM). mRNA levels from these cells were analyzed by real-time RT-PCR. Standard protocol was followed for immunofluorescence detection of ionic transporters. The lacrimal duct system was divided into six segments on the basis of morphologic characteristics: the intercalated, intralobular, interlobular, intralobar, interlobar, and main excretory ducts. Although the morphologic features change incrementally along the entire duct system, the gene expression of ionic transporters and aquaporins, including AE3, AQP4, AQP5, CFTR, ClC2gamma, KCC1, NHE1, NKAalpha1, NKAbeta1, NKAbeta2, NKAbeta3, and NKCC1 varied greatly among duct segments. Immunofluorescence results were g...Continue Reading

Citations

Jul 21, 2012·American Journal of Physiology. Cell Physiology·Dongfang YuRichard C Boucher
Mar 21, 2013·Investigative Ophthalmology & Visual Science·Shivaram SelvamSamuel C Yiu
Jul 23, 2014·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Amina B El-FadalyDoaa M A Shuaib
May 15, 2012·Current Eye Research·Michael Lu, Chuanqing Ding
Nov 4, 2015·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Laura LossiAdalberto Merighi
Sep 27, 2012·Current Eye Research·Jianyan HuangChuanqing Ding
Apr 29, 2011·Current Eye Research·Chuanqing DingYanru Wang
Nov 5, 2013·Biochimica Et Biophysica Acta·Kevin L ScheyYing Qi
Jan 6, 2015·The Ocular Surface·Austin K MircheffJoel E Schechter
Nov 14, 2013·The Journal of Craniofacial Surgery·Jung Wan Kim, Sehyun Baek
Dec 26, 2012·Endocrinology·Charles Pyke, Lotte Bjerre Knudsen
May 24, 2017·Molecular Medicine Reports·Hongwei WangXin Wang
Jun 25, 2021·Frontiers in Pharmacology·Maurizio CammalleriDario Rusciano

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