Ductular cell proliferation in islet cell neogenesis induced by incomplete ligation of the pancreatic duct in dogs

Surgery Today
Masaki NagayaKatsuya Akashi

Abstract

It has been suggested that islet neogenesis can be induced by incomplete ligation of the pancreatic duct in small animals; however, there has been no report of neogenesis and the proliferation of islets occurring in larger animals. When this procedure was performed in the Vervet monkey, it produced a noticeable increase in duct proliferation, but islet neogenesis was not observed, although the number of monkeys examined was very small. We conducted this study to evaluate whether islet neogenesis and ductular proliferation could be induced in larger animals such as the dog, by partial obstruction of the pancreatic duct. Incomplete ligation of the pancreatic duct was induced by tying the pancreas around the ventral side of the head with 2-0 silk and reducing the circumference by about 80% to cause partial obstruction. By 2 weeks after ligation, we saw hyperplasia of the epithelial cells, multilayering of cuboidal cells, and proliferation of ductular cells. The terminal ductules involved in the formation of immunohistochemically insulin-positive islets, and islets, formed adjacent to the alignment of the ductular cells. By 8 weeks after ligation we saw scattered islets, less than 50 micro m in diameter and less than 1 000 microm(2...Continue Reading

Citations

Jun 13, 2008·Clinical Reviews in Allergy & Immunology·Marco MarzioniAntonio Benedetti
Jun 22, 2011·Journal of Veterinary Internal Medicine·P J WatsonM E Herrtage
Feb 20, 2010·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·Marco MarzioniAntonio Benedetti
Jan 15, 2015·The Journal of Small Animal Practice·P Watson
Jul 28, 2011·Pancreas·Orison O WoolcottRobert H Chow
May 11, 2019·PloS One·Masaki NagayaHiroshi Nagashima

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