Duodenal expression of the epithelial calcium transporter gene TRPV6: is there evidence for Vitamin D-dependence in humans?

The Journal of Steroid Biochemistry and Molecular Biology
Julian R F WaltersOrli Rhodes-Kendler

Abstract

Intestinal absorption of dietary calcium is regulated by 1,25-dihydroxycholecalciferol (1,25(OH)(2)D(3)) in humans and in experimental animals but interspecies differences in responsiveness to 1,25(OH)(2)D(3) are found, possibly due to differences in the promoters of genes for intestinal calcium transport proteins or of the Vitamin D receptor (VDR). The epithelial calcium transporter, known as ECAC2 or CAT1, the product of the TRPV6 gene expressed in proximal intestinal enterocytes, is the first step in calcium absorption and studies in mice have shown that its expression is Vitamin D-dependent. In contrast in man, we showed that duodenal TRPV6 mRNA expression was independent of blood 1,25(OH)(2)D(3), although in Caco-2 cells, 1,25(OH)(2)D(3)-dependent changes have been demonstrated. We sought to explain these findings. A consensus Vitamin D response element in the mouse gene is absent in the human gene. We re-analysed our duodenal expression data according to a CDX2-site polymorphism in the VDR promoter. Mean TRPV6 expression was the same, but there was evidence of different responsiveness to 1,25(OH)(2)D(3). In the GG genotype group, but not the AG, duodenal TRPV6 expression increased with 1,25(OH)(2)D(3). We postulate that l...Continue Reading

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Citations

Apr 12, 2005·Archives of Biochemistry and Biophysics·Alex J BrownH James Armbrecht
Dec 5, 2008·Nutrition Reviews·Evelyne van EttenLut Overbergh
Aug 11, 2012·Endocrinology and Metabolism Clinics of North America·Ronald D Emkey, Gregory R Emkey
Oct 19, 2006·The Journal of Steroid Biochemistry and Molecular Biology·Masaki NakaneJ Ruth Wu-Wong
Sep 28, 2006·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Julian R F WaltersRichard Poulsom
Jun 7, 2007·The Journal of Biological Chemistry·V'yacheslav Lehen'kyiNatalia Prevarskaya
Apr 5, 2011·Archives of Biochemistry and Biophysics·Viviana CentenoNori Tolosa de Talamoni

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