Duplex functional G-quadruplex/NMM fluorescent probe for label-free detection of lead(II) and mercury(II) ions

Journal of Hazardous Materials
Qian ZhuXiaohong Zhou

Abstract

To meet the severe water pollution status, multi-target biosensor becomes one of research focus. We herein report a novel duplex functional fluorescent biosensor for the label-free and separate detection of Pb2+ and Hg2+ with high sensitivity and selectivity. A K+-induced fluorescent G-quadruplex probe was assembled by a guanine-rich sequence (AGRO100) and N-methyl mesoporphyrin IX(NMM). It changed into a more stably non-fluorescent G-quadruplex structure and a hairpin-like structure upon binding Pb2+ and Hg2+ ions, respectively. As a result, the fluorescence decreased with relation to the addition of targets, allowing the separate detection of Pb2+ and Hg2+ ions at concentrations as low as 5 nM and 18.6 nM. The linear correlation existed between the fluorescence intensity and the concentration of Pb2+ and Hg2+ over the range of 10-200 nM (R2 = 0.98) and 20-1000 nM (R2 = 0.99), respectively. All real lake samples, even containing Pb2+ or Hg2+ at 50 nM, could be determined by the duplex functional fluorescent probe with recovery rates 96% and 104%, respectively. Considering that this sensor is label-free, simple, time-saving, cost-effective and easy-to-handle, it is possible to pave its way for Pb2+ and Hg2+ detection in various...Continue Reading

Citations

Mar 19, 2020·Chembiochem : a European Journal of Chemical Biology·Karolis NorvaišaMathias O Senge
Dec 21, 2020·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Yamin YuBaocun Zhu
Jan 25, 2021·Analytica Chimica Acta·Yongjie YangJuewen Liu
Apr 4, 2021·Biosensors·Kasinee KatelakhaWanida Laiwattanapaisal
Dec 1, 2019·Journal of Porphyrins and Phthalocyanines·Ariana YettLiliya A Yatsunyk

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved