PMID: 2110820Mar 27, 1990Paper

Duplex oligodeoxyribonucleotides cross-linked by mitomycin C at a single site: synthesis, properties, and cross-link reversibility

Biochemistry
H Borowy-BorowskiMaria Tomasz

Abstract

Oligodeoxyribonucleotides cross-linked by reductively activated mitomycin C (MC) were prepared and purified for the first time. The cross-linked products were structurally characterized by nucleoside and MC-nucleoside adduct analysis. Optimal conditions were established for the cross-linking reaction, resulting in high yields, typically in the 20-50% range. Nuclease digests of the cross-linked oligonucleotides yielded the same bifunctional MC-deoxyguanosine adduct as that previously isolated from DNA exposed to MC in vitro and in vivo [Tomasz et al. (1987) Science 235, 1204]. The cross-linked oligonucleotides displayed broad thermal melting profiles, greatly increased Tm, and complex circular dichroism spectra. Phosphodiester linkages at the cross-link were resistant to spleen exonuclease, nuclease P1, and TaqI and ClaI restriction endonucleases; snake venom diesterase action was uninhibited. The cross-links are stable to heat at neutral pH but are removed by treatment in hot piperidine or by the reducing agents Na2S2O4 and dithiothreitol. Mechanisms are proposed for these reactions. These studies define optimal methods for introducing mitomycin cross-links into DNA fragments at a specific site, providing a versatile tool to st...Continue Reading

References

May 2, 1989·Biochemistry·S P TengD M Crothers
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M TomaszG L Verdine
Jul 15, 1986·Biochemistry·D M Peterson, J Fisher
Dec 1, 1988·Journal of Biomolecular Structure & Dynamics·A K Chawla, M Tomasz
Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·K RanderathR C Gupta

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Citations

Feb 1, 1994·Molecular & General Genetics : MGG·J Zakrzewska-CzerwińskaH Schrempf
Nov 3, 2004·Journal of the American Society for Mass Spectrometry·Yuesong WangYinsheng Wang
Jan 11, 1994·Nucleic Acids Research·A AsaiH Nakano
Jul 22, 2010·Nucleic Acids Research·Mao-wen WengMoon-shong Tang
May 22, 2007·Chemico-biological Interactions·David J MustraJoshua W Hamilton
Mar 10, 1992·Biochemistry·M A WarpehoskiT J Monroe
Nov 2, 2016·Journal of Bacteriology·Peter E Burby, Lyle A Simmons
Nov 1, 2018·Molecular Microbiology·Peter E Burby, Lyle A Simmons
Feb 17, 1998·Environmental and Molecular Mutagenesis·A J WarrenJ W Hamilton
Jun 29, 2001·Journal of Applied Toxicology : JAT·S I BaskinM J Novak
Feb 19, 2002·Chemical Reviews·Scott R. Rajski, Robert M. Williams
Jul 29, 2021·Chemistry : a European Journal·Owen ZacariasElise Champeil

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