Duplicated or Hybridized Peptide Functional Domains Promote Oral Homeostasis

Journal of Dental Research
T BasiriWalter L Siqueira

Abstract

Proteins that have existed for millions of years frequently contain repeats of functional domains within their primary structure, thereby improving their functional capacity. In the evolutionary young statherin protein contained within the in vivo-acquired enamel pellicle (AEP), we identified a single functional domain (DR9) located within the protein N-terminal portion that exhibits a higher affinity for hydroxyapatite and more efficient protection against enamel demineralization compared to other native statherin peptides. Thus, we tested the hypothesis that multiplication of functional domains of naturally occurring pellicle peptides amplifies protection against enamel demineralization. In addition, a specific amino acid sequence from histatin 3 (RR-14) was introduced to the hybrid peptides for further testing. Enamel specimens were sectioned to 150-µm thickness and randomly grouped as follows: DR9, DR9-DR9, DR9-RR14, statherin, histatin 1, or distilled water (control). After submersion for 2 h at 37°C, the specimens were placed in 2 mL demineralization solution for 12 d at 37°C. Upon sample removal, the remaining solution was subjected to colorimetric assays to determine the amount of calcium and phosphate released from eac...Continue Reading

References

Jan 1, 1977·Archives of Oral Biology·R T Zahradnik, E C Moreno
Aug 1, 1991·Infection and Immunity·T XuF G Oppenheim
Jan 1, 1993·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·L A BobekM J Levine
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·J R LongG P Drobny
Feb 15, 2002·Advances in Dental Research·M Edgerton, S E Koshlukova
Nov 18, 2004·Journal of Applied Microbiology·J LiF G Oppenheim
Feb 9, 2006·Journal of Child and Adolescent Psychiatric Nursing : Official Publication of the Association of Child and Adolescent Psychiatric Nurses, Inc·Kathleen M ReganLee Vorderer
Aug 12, 2006·Archives of Oral Biology·E J HelmerhorstF G Oppenheim
Feb 17, 2007·Annals of the New York Academy of Sciences·Frank G OppenheimEva J Helmerhorst
Apr 24, 2007·Journal of Proteome Research·Walter L SiqueiraFrank G Oppenheim
May 23, 2007·Biomedical Chromatography : BMC·Rui VitorinoFrancisco M L Amado
Jul 27, 2007·Journal of Dental Research·E J Helmerhorst, F G Oppenheim
Aug 16, 2008·Cells, Tissues, Organs·Graeme K HunterHarvey A Goldberg
Mar 10, 2009·Archives of Oral Biology·Walter L Siqueira, Frank G Oppenheim
Feb 23, 2010·PloS One·Paul V AzzopardiGraeme K Hunter
Mar 31, 2010·Journal of Dental Research·W L SiqueiraF G Oppenheim
Jul 16, 2010·Dental Clinics of North America·Anderson T Hara, Domenick T Zero
Nov 16, 2010·Journal of Dental Research·E E McDonaldW L Siqueira
Sep 20, 2011·Journal of Cell Communication and Signaling·Dusa VukosavljevicWalter L Siqueira
Sep 29, 2012·Journal of Dental Research·W L SiqueiraE E McDonald
Jun 28, 2015·Journal of Dental Research·Y XiaoW L Siqueira

❮ Previous
Next ❯

Citations

Mar 2, 2018·Scientific Reports·Maria Teresa ValenteWalter Luiz Siqueira
Jan 9, 2021·Archives of Oral Biology·John Yun NiuChun Hung Chu
May 1, 2021·Nanomaterials·Yi ZhuWalter L Siqueira
Jul 26, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Fan ZhangJianshu Li

❮ Previous
Next ❯

Methods Mentioned

BETA
Assay

Software Mentioned

Minitab

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.