Duplication and selection in β-ketoacyl-ACP synthase gene lineages in the sexually deceptive Chiloglottis (Orchidaceace)

Annals of Botany
Darren C J WongRod Peakall

Abstract

The processes of gene duplication, followed by divergence and selection, probably underpin the evolution of floral volatiles crucial to plant-insect interactions. The Australian sexually deceptive Chiloglottis orchids use a class of 2,5-dialkylcyclohexan-1,3-dione volatiles or 'chiloglottones' to attract specific male wasp pollinators. Here, we explore the expression and evolution of fatty acid pathway genes implicated in chiloglottone biosynthesis. Both Chiloglottis seminuda and C. trapeziformis produce chiloglottone 1, but only the phylogenetically distinct C. seminuda produces this volatile from both the labellum callus and glandular sepal tips. Transcriptome sequencing and tissue-specific contrasts of the active and non-active floral tissues was performed. The effects of the fatty acid synthase inhibitor cerulenin on chiloglottone production were tested. Patterns of selection and gene evolution were investigated for fatty acid pathway genes. Tissue-specific differential expression of fatty acid pathway transcripts was evident between active and non-active floral tissues. Cerulenin significantly inhibits chiloglottone 1 production in the active tissues of C. seminuda. Phylogenetic analysis of plant β-ketoacyl synthase I (KAS...Continue Reading

References

Mar 1, 1992·Molecular and Cellular Endocrinology·H M TargovnikG Vassart
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Feb 17, 2005·BMC Bioinformatics·Guy St C Slater, Ewan Birney
May 3, 2005·Annual Review of Plant Biology·David R Gang
Feb 14, 2006·Science·Eran PicherskyNatalia Dudareva
May 8, 2007·Molecular Biology and Evolution·Ziheng Yang
Jul 7, 2007·Molecular Ecology·Rod Peakall
Aug 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Patrik JohanssonMartin Grininger
May 28, 2009·Proceedings of the National Academy of Sciences of the United States of America·S FrankeW Francke
Jun 27, 2009·The New Phytologist·Lex E Flagel, Jonathan F Wendel
Dec 17, 2009·BMC Bioinformatics·Christiam CamachoThomas L Madden
Mar 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Philipp M SchlüterFlorian P Schiestl
May 17, 2011·Nature Biotechnology·Manfred G GrabherrAviv Regev
Feb 9, 2012·Proceedings of the National Academy of Sciences of the United States of America·Ruiqi HuangTodd J Barkman
Aug 24, 2012·PLoS Genetics·Shuqing XuFlorian P Schiestl
Feb 18, 2014·Bioinformatics·Yinlong XieJun Wang
Apr 4, 2014·Bioinformatics·Anthony M BolgerBjoern Usadel
Jun 20, 2014·Nature Communications·Nethaji J GallageBirger Lindberg Møller
Sep 23, 2014·Functional & Integrative Genomics·Alok K GuptaFlorian P Schiestl
Nov 6, 2014·Molecular Biology and Evolution·Lam-Tung NguyenBui Quang Minh
Nov 25, 2014·Nature Genetics·Jing CaiZhong-Jian Liu
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
Feb 5, 2015·Annals of Botany·Ranamalie AmarasingheRod Peakall
Mar 10, 2015·Nature Methods·Daehwan KimSteven L Salzberg
Aug 28, 2015·Proceedings. Biological Sciences·Thomas J GivnishKenneth M Cameron
May 24, 2016·Current Biology : CB·Khalid E M SedeekPhilipp M Schlüter
Jun 12, 2016·Plant Physiology·Nicholas PanchyShin-Han Shiu
Jul 2, 2016·Current Opinion in Plant Biology·Björn BohmanRod Peakall
Sep 9, 2016·The New Phytologist·Eran Pichersky, Robert A Raguso

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