Dural administration of inflammatory soup or Complete Freund's Adjuvant induces activation and inflammatory response in the rat trigeminal ganglion

The Journal of Headache and Pain
M LukácsLars Edvinsson

Abstract

Migraine is a painful disorder with a huge impact on individual and public health. We hypothesize that migraine pain originates from a central mechanism that results secondarily in hypersensitivity in peripheral afferents associated with the cerebral and cranial blood vessels. It has previously been shown that application of inflammatory or algesic substances onto the dura mater or chemical stimulation of the dural receptive fields causes hypersensitivity to mechanical and thermal stimulation together with direct activation of the TG. We asked whether local inflammation of dura mater induces inflammatory activation in the trigeminal ganglion. We performed topical administration of inflammatory soup (IS) or Complete Freund's Adjuvant (CFA) onto an exposed area of the rat dura mater in vivo for 20 min. The window was closed and the rats were sacrificed after 4 h and up to 7 days. Myography was performed on middle meningeal arteries. The trigeminal ganglia were removed and processed for immunohistochemistry or Western blot. Both CFA and IS induced enhanced expression of pERK1/2, IL-1β and CGRP in the trigeminal ganglia. The pERK1/2 immunoreactivity was mainly seen in the satellite glial cells, while IL-1β reactivity was observed i...Continue Reading

References

Apr 1, 1990·Journal of Neurophysiology·E LangH O Handwerker
Aug 1, 1995·Scandinavian Journal of Clinical and Laboratory Investigation·E HelsetJ M Lundberg
Aug 11, 2001·Journal of Neuroimmunology·J C CoprayH W Boddeke
Nov 17, 2005·Journal of Neuroinflammation·Saku RuohonenMatias Röyttä
Feb 24, 2006·Journal of Neurophysiology·Andrew M Strassman, Dan Levy
Aug 25, 2006·Headache·Paul L Durham
Jan 16, 2007·Brain, Behavior, and Immunity·Pavel Dublin, Menachem Hanani
Oct 30, 2007·Nature Neuroscience·Joachim Scholz, Clifford J Woolf
Jan 25, 2008·The Journal of Headache and Pain·Andrea Stephanie LinkLars Edvinsson
Jul 23, 2008·Cephalalgia : an International Journal of Headache·W F StewartUNKNOWN AMPP Advisory Group
Jan 27, 2009·Brain Research Reviews·Ke Ren, Richard Torres
Jun 23, 2009·Lancet Neurology·Jes OlesenPeer Tfelt-Hansen
Sep 22, 2010·Cephalalgia : an International Journal of Headache·Lars EdvinssonAntoinette MaassenVanDenBrink
Oct 27, 2010·Cephalalgia : an International Journal of Headache·Troels WieneckeMessoud Ashina
Feb 1, 2011·Cephalalgia : an International Journal of Headache·Messoud AshinaJes Olesen
Jun 2, 2012·Cephalalgia : an International Journal of Headache·Jan HoffmannUwe Reuter
Oct 15, 2013·The European Journal of Neuroscience·Sonu BhaskarHoushang Amiri
Mar 4, 2015·British Journal of Clinical Pharmacology·Lars Edvinsson

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Citations

May 18, 2016·The Journal of Headache and Pain·Mária DuxGábor Jancsó
Dec 19, 2017·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Simona D FrederiksenLars Edvinsson
Nov 20, 2018·Journal of Neuroscience Research·Wenguo FanHongwen He
Apr 16, 2019·Headache·Smriti IyengarSheena K Aurora
Jul 3, 2019·Nature Reviews. Neurology·Lars EdvinssonKarin Warfvinge
May 1, 2019·The Journal of Headache and Pain·Tamás KörtésiJános Tajti
Jun 1, 2018·Current Medicinal Chemistry·Laszlo VécseiLars Edvinsson
Dec 25, 2019·The Journal of Headache and Pain·Anna P Andreou, Lars Edvinsson
Aug 18, 2020·The Journal of Headache and Pain·Klaudia Flóra LaborcÁrpád Párdutz
Aug 28, 2020·International Journal of Molecular Sciences·Filip JovanovicNebojsa Nick Knezevic
Apr 25, 2018·Nature Reviews. Neurology·Lars EdvinssonDiana N Krause
Apr 12, 2019·Pharmaceuticals·Silvia Benemei, Greg Dussor
Jun 7, 2020·The Journal of Headache and Pain·J C A EdvinssonUNKNOWN European Headache Federation School of Advanced Studies (EHF-SAS)
Apr 17, 2019·CNS Drugs·Kristian Agmund Haanes, Lars Edvinsson
Aug 30, 2019·The Journal of Headache and Pain·Andrea M HarriottPhilip R Holland
Nov 15, 2020·Journal of Neuroscience Methods·Cynthia AbboudMarc Landry
Jun 3, 2021·International Journal of Molecular Sciences·Yi YeJennifer Gibbs
Nov 23, 2021·Headache·Andrew CharlesPeter Goadsby

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Methods Mentioned

BETA
protein assay
electrophoresis
dissection

Software Mentioned

Adobe Photoshop CS3
ImageJ

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