PMID: 8604331Mar 15, 1996Paper

During in vivo maturation of eukaryotic nuclear mRNA, splicing yields excised exon circles

Nucleic Acids Research
B Bailleul

Abstract

Circular splicing has already been described on nuclear pre-mRNA for certain splice sites far apart in the multi exonic ETS-1 gene and in the single 1.2 kb exon of the Sry locus. To date, it is unclear how splice site juxtaposition occurs in normal and circular splicing. The splice site selection of an internal exon is likely to involve pairing between splice sites across that exon. Based on this, we predict that, albeit at low frequency, internal exons yield circular RNA by splicing as an error-prone mechanism of exon juxtaposition or, perhaps more interestingly, as a regulated mechanism on alternative exons. To address this question, the circular exon formation was analyzed at three ETS-1 internal exons (one alternative spliced exon and two constitutive), in human cell line and blood cell samples. Here, we show by RT-PCR and sequencing that exon circular splicing occurs at the three individual exons that we examined. RNase protection experiments suggest that there is no correlation between exon circle expression and exon skipping.

References

Oct 25, 1992·Nucleic Acids Research·M Puttaraju, M D Been
Mar 1, 1992·The EMBO Journal·C CocquerelleB Bailleul
Oct 25, 1992·Nucleic Acids Research·L QianM F Wilkinson
Jan 1, 1991·Annual Review of Cell Biology·M R Green
Feb 8, 1991·Cell·J M NigroB Vogelstein
Nov 11, 1988·Nucleic Acids Research·J D Hawkins
Jan 1, 1988·Journal of Molecular Evolution·M W Smith
Jun 4, 1974·Biochemistry·V GlisinC Byus
Jan 22, 1982·Nucleic Acids Research·S M Mount
Apr 11, 1995·Nucleic Acids Research·C A Schindewolf, H Domdey
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K A Jarrell
Jan 15, 1993·European Journal of Biochemistry·B WasylykA Giovane

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Citations

Sep 17, 2013·PLoS Genetics·Julia SalzmanPatrick O Brown
Mar 13, 2014·PloS One·Peter L WangJulia Salzman
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·S A FrantzN J Samani
May 29, 2014·International Journal of Molecular Sciences·Hitoshi Suzuki, Toshifumi Tsukahara
Apr 7, 2016·Trends in Genetics : TIG·Julia Salzman
Jun 24, 2008·Molecular Cell·Duncan J SmithMaria M Konarska
Sep 13, 2016·Frontiers in Molecular Neuroscience·Daniëlle van RossumR Jeroen Pasterkamp
Oct 16, 2016·Blood Cancer Journal·A BonizzatoS Bortoluzzi
Jul 18, 2018·Acta Pharmacologica Sinica·Guo-Yi JiaPeng Wang
Aug 23, 2020·International Journal of Molecular Sciences·Michaela Dostalova MerkerovaDavid Kundrat
Jun 22, 2021·Frontiers in Cell and Developmental Biology·Dean RaoWenjie Huang
Aug 13, 2021·Nature Reviews. Nephrology·Anton Jan van ZonneveldJohan M Lorenzen

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