During Infection, Theiler's Virions Are Cleaved by Caspases and Disassembled into Pentamers

Journal of Virology
Sevim Yildiz ArslanHoward L Lipton

Abstract

Infected macrophages in spinal cords of mice persistently infected with Theiler's murine encephalomyelitis virus (TMEV) undergo apoptosis, resulting in restricted virus yields, as do infected macrophages in culture. Apoptosis of murine macrophages in culture occurs via the intrinsic pathway later in infection (>10 h postinfection [p.i.]) after maximal virus titers (150 to 200 PFU/cell) have been reached, with loss of most infectious virus (<5 PFU/cell) by 20 to 24 h p.i. Here, we show that BeAn virus RNA replication, translation, polyprotein processing into final protein products, and assembly of protomers and pentamers in infected M1-D macrophages did not differ from those processes in TMEV-infected BHK-21 cells, which undergo necroptosis. However, the initial difference from BHK-21 cell infection was seen at 10 to 12 h p.i., where virions from the 160S peak in sucrose gradients had incompletely processed VP0 (compared to that in infected BHK-21 cells). Thereafter, there was a gradual loss of the 160S virion peak in sucrose gradients, with replacement by a 216S peak that was observed to contain pentamers among lipid debris in negatively stained grids by electron microscopy. After infection or incubation of purified virions wit...Continue Reading

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Citations

May 20, 2017·Cell Death and Differentiation·Patrick F Connolly, Howard O Fearnhead
May 3, 2019·Cell Death and Differentiation·Rochelle TixeiraIvan K H Poon
Jan 24, 2019·International Journal of Molecular Sciences·Ingo GerhauserAndreas Beineke

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