Dusky-like is required for epidermal pigmentation and metamorphosis in Tribolium castaneum

Scientific Reports
Chengjun LiBin Li

Abstract

Dusky-like (Dyl) is associated with the morphogenesis of embryonic denticle, adult sensory bristle and wing hair in Drosophila melanogaster. And whether Dyl involved in insect post-embryonic development and its signal transduction are poorly understood. Here, phylogenetic analysis revealed that dyl displayed one-to-one orthologous relationship among insects. In Tribolium castaneum, dyl is abundantly expressed at the late embryonic stage. Tissue-specific expression analysis at the late adult stage illustrated high expression of dyl in the fat body and ovary. Knockdown of dyl resulted in the defects in larval epidermal pigmentation and completely blocked the transitions from larval to pupal and pupal to adult stages of T. castaneum. We further discovered that dyl RNAi phenotypes were phenocopied by blimp-1 or shavenbaby (svb) silencing, and dyl was positively regulated by blimp-1 through svb in T. castaneum. These results suggest that Dyl functions downstream of Blimp-1 through Svb for larval epidermal pigmentation and metamorphosis. Moreover, ftz-f1 was down-regulated after RNA interference (RNAi) suppressing any of those three genes, indicating that Ftz-f1 works downstream of Dyl to mediate the effects of Blimp-1, Svb and Dyl o...Continue Reading

References

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Dec 3, 2014·Insect Biochemistry and Molecular Biology·Xin-Ping LiuGuo-Qing Li

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Citations

Jul 10, 2018·Archives of Insect Biochemistry and Physiology·Chengjun LiKeping Chen
Feb 3, 2021·Current Opinion in Genetics & Development·Sebastian KittelmannNicolás Frankel

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Datasets Mentioned

BETA
TC001440
TC002550
TC008099
TC006673
TC005627

Methods Mentioned

BETA
PCR

Software Mentioned

MEGA
Miniature
SPSS
Dy
Dyl
Clustal Omega

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