dUTPase activity is critical to maintain genetic stability in Saccharomyces cerevisiae.

Nucleic Acids Research
Marie GuilletSerge Boiteux

Abstract

We identified a viable allele (dut1-1) of the DUT1 gene that encodes the dUTPase activity in Saccharomyces cerevisiae. The Dut1-1 protein possesses a single amino acid substitution (Gly82Ser) in a conserved motif nearby the active site and exhibits a greatly reduced dUTPase activity. The dut1-1 single mutant exhibits growth delay and cell cycle abnormalities and shows a strong spontaneous mutator phenotype. All phenotypes of the dut1-1 mutant are suppressed by the simultaneous inactivation of the uracil DNA N-glycosylase, Ung1. However, the ung1 dut1-1 double mutant accumulates uracil in its genomic DNA. The viability of the dut1-1 mutant is greatly impaired by the simultaneous inactivation of AP endonucleases. These data strongly suggest that the phenotypes of the dut1-1 mutant result from the incorporation of dUMPs into DNA subsequently converted into AP sites. The analysis of the dut1-1 strain mutation spectrum showed that cytosines are preferentially incorporated in front of AP sites in a Rev3-dependent manner during translesion synthesis. These results point to a critical role of the Dut1 protein in the maintenance of the genetic stability. Therefore, the normal cellular metabolism, and not only its byproducts, is an impor...Continue Reading

References

Feb 27, 1979·Biochimica Et Biophysica Acta·D K DubeL A Loeb
Jan 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·B K TyeB Weiss
Mar 25, 1992·Nucleic Acids Research·D GietzR H Schiestl
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J E Kranz, C Holm
Jul 7, 1999·The EMBO Journal·M OtterleiH E Krokan
Apr 25, 2000·Molecular and Cellular Biology·C A Torres-RamosS Prakash
Jul 27, 2002·Science·John Rouse, Stephen P Jackson
Oct 31, 2003·Molecular and Cellular Biology·Marie Guillet, Serge Boiteux
Feb 26, 2004·Molecular Microbiology·Elena A Kouzminova, Andrei Kuzminov
Jun 23, 2004·The Journal of Biological Chemistry·Orsolya BarabásBeáta G Vértessy
Dec 1, 2004·Annual Review of Genetics·Deborah E Barnes, Tomas Lindahl
Nov 1, 2005·Nucleic Acids Research·Yoke W KowMarc M Greenberg
Nov 30, 2005·Proceedings of the National Academy of Sciences of the United States of America·Paul AuerbachBruce Demple
Jun 24, 2006·Methods in Enzymology·Serge Boiteux, Marie Guillet

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Citations

May 26, 2007·Journal of Biomedical Science·Chun-Mei Hu, Zee-Fen Chang
May 19, 2009·Nature·Nayun Kim, Sue Jinks-Robertson
Sep 12, 2013·Proceedings of the National Academy of Sciences of the United States of America·Paulina ProrokMurat K Saparbaev
Jan 9, 2010·The Journal of Biological Chemistry·Dolores Córdoba-CañeroTeresa Roldán-Arjona
Dec 8, 2010·Molecular Biology and Evolution·J Ignacio Lucas-LledóMichael Lynch
May 5, 2011·PloS One·Emeline DuboisMarie-Pascale Doutriaux
Jun 2, 2012·PloS One·Ildiko PecsiJudit Tóth
Jun 26, 2012·The International Journal of Biochemistry & Cell Biology·Víctor M Castillo-AcostaDolores González-Pacanowska
Sep 7, 2011·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·W H YapY M Lim
Mar 17, 2010·DNA Repair·Nicolas SiaudMarie-Pascale Doutriaux
Jun 6, 2009·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Leontina Di CeccoSilvia Pellegrini
Jul 29, 2008·The International Journal of Biochemistry & Cell Biology·Víctor M Castillo-AcostaDolores González-Pacanowska
Apr 27, 2007·Biochimie·Ramiro Téllez-SanzLuis García-Fuentes
Mar 5, 2015·The FEBS Journal·András HorváthBeáta G Vértessy
May 10, 2011·The Biochemical Journal·Anatoli TchigvintsevAlexander F Yakunin
Dec 4, 2012·Molecular and Biochemical Parasitology·Víctor M Castillo-AcostaDolores González-Pacanowska
Dec 15, 2010·Biochimica Et Biophysica Acta·Drew WilliamsMichael T Greenwood
Apr 24, 2009·FEMS Microbiology Reviews·Tiago Dos VultosBrigitte Gicquel
Jul 19, 2011·Tuberculosis·Krishna Kurthkoti, Umesh Varshney
Jul 30, 2016·PloS One·Sabrina WemhoffFriedhelm Meinhardt
Feb 13, 2016·Microbiology and Molecular Biology Reviews : MMBR·Monika SchmollAlfredo Herrera-Estrella
Nov 7, 2008·Journal of Radiation Research·Shin-Ichiro YonekuraQiu-Mei Zhang-Akiyama
Apr 3, 2013·Genetics·Serge Boiteux, Sue Jinks-Robertson
Sep 24, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Benjamin RocheFrançois Rougeon
Aug 5, 2019·Cellular and Molecular Life Sciences : CMLS·Blanca Martínez-ArribasDolores González-Pacanowska
Oct 27, 2015·G3 : Genes - Genomes - Genetics·Xiaoge GuoSue Jinks-Robertson
Oct 28, 2020·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Fei GaoYing Li
Dec 22, 2020·Frontiers in Cell and Developmental Biology·Noha ElsakrmyDindial Ramotar
May 1, 2021·International Journal of Molecular Sciences·Ryszard OlinskiHans Einar Krokan
Aug 27, 2021·Metabolites·Kristina J H KleinwortCornelia A Deeg
Jul 13, 2014·Genome Research·D Suzi BryanJay R Hesselberth

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Methods Mentioned

BETA
dissection
PCR
FACS
deamination

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