dye (arc) Mutants: insights into an unexplained phenotype and its suppression by the synthesis of poly (3-hydroxybutyrate) in Escherichia coli recombinants

FEMS Microbiology Letters
Jimena A RuizM J Pettinari

Abstract

arcA codes for a central regulator in Escherichia coli that responds to redox conditions of growth. Mutations in this gene, originally named dye, confer sensitivity to toluidine blue and other redox dyes. However, the molecular basis for the dye-sensitive phenotype has not been elucidated. In this work, we show that toluidine blue redirects electrons to O2 and causes an increase in the generation of reactive O2 species (ROS). We also demonstrate that synthesis of poly (3-hydroxybutyrate) suppresses the Dye phenotype in E. coli recombinants, as the capacity to synthesize the polymer reduces sensitivity to toluidine blue, O2 consumption and ROS production levels.

References

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Citations

Oct 16, 2012·Applied and Environmental Microbiology·Gonzalo N BidartPablo I Nikel
Jan 19, 2010·Applied and Environmental Microbiology·Alejandra de AlmeidaM Julia Pettinari
Mar 20, 2008·Journal of Molecular Microbiology and Biotechnology·M Julia PettinariBeatriz S Méndez
Mar 20, 2008·Journal of Molecular Microbiology and Biotechnology·Pablo I NikelBeatriz S Méndez
Jun 26, 2007·Molecular Microbiology·Jeffrey L BoseEric V Stabb
Sep 14, 2016·Photodiagnosis and Photodynamic Therapy·Lautaro GándaraAdriana Casas
Oct 27, 2015·Applied and Environmental Microbiology·Manuel S GodoyM Julia Pettinari
Nov 2, 2014·BMC Microbiology·Daniel C LimaSilvia R Batistuzzo de Medeiros

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