Dye attached poly(hydroxyethyl methacrylate) cryogel for albumin depletion from human serum

Journal of Separation Science
Muge AndacA Denizli

Abstract

Cibacron Blue F3GA was immobilized on poly(hydroxyethyl methacrylate) cryogel and it was used for selective and efficient depletion of albumin from human serum. The poly(hydroxyethyl methacrylate) was selected as the basic component because of its inertness, mechanical strength, chemical and biological stability, and biocompatibility. Cibacron Blue F3GA was covalently attached to the poly(hydroxyethyl methacrylate) cryogel to produce poly(hydroxyethyl methacrylate)-Cibacron Blue F3GA cryogel affinity column. The poly(hydroxyethyl methacrylate)-Cibacron Blue F3GA cryogel was characterized with respect to gelation yield, swelling degree, total volume of macropores, Fourier Transform Infrared spectroscopy, and scanning electron microscopy. It was found that the maximum amount of adsorption (343 mg/g of dry cryogel) obtained from experimental results is very close to the calculated Langmuir adsorption capacity (345 mg/g of dry cryogel). The maximum adsorption capacity for poly(hydroxyethyl methacrylate)-Cibacron Blue F3GA cryogel column was obtained as 950 mg/g of dry cryogel for nondiluted serum. The adsorption capacity decreased with increasing dilution ratios while the depletion ratio of albumin remained as 77% in serum sample. ...Continue Reading

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Citations

Nov 29, 2012·Analytical and Bioanalytical Chemistry·Erika L PfaunmillerDavid S Hage
Jul 11, 2013·Applied Biochemistry and Biotechnology·Işık PerçinAdil Denizli
Jan 11, 2013·Twin Research and Human Genetics : the Official Journal of the International Society for Twin Studies·Athula SumathipalaDinesha Gunewardane
Jan 12, 2013·Artificial Cells, Nanomedicine, and Biotechnology·Gözde Baydemir, Mehmet Odabaşı
Feb 18, 2014·Artificial Cells, Nanomedicine, and Biotechnology·Murat UygunAdil Denizli
Oct 8, 2013·Artificial Cells, Nanomedicine, and Biotechnology·Öznur GökayAdil Denizli
Oct 12, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Müge AndaçAdil Denizli
Feb 15, 2017·Journal of Separation Science·Binnaz DemirciAdil Denizli
Dec 3, 2014·Colloids and Surfaces. B, Biointerfaces·Deniz Türkmen, Adil Denizli
Oct 12, 2018·Journal of Biomaterials Science. Polymer Edition·Esra Feyzioğlu DemirAdil Denizli
Oct 31, 2013·Artificial Cells, Nanomedicine, and Biotechnology·Müge Andaç
May 16, 2014·Journal of Separation Science·Fatma Kartal, Adil Denizli
Jun 4, 2019·Journal of Biomaterials Science. Polymer Edition·Gizem UzunoğluAdil Denizli
Jun 3, 2016·Materials·Robert J Groarke, Dermot Brabazon
Jun 14, 2014·Biotechnology and Applied Biochemistry·Gözde BaydemirAdil Denizli
Jun 12, 2014·Journal of Chromatography. a·Gizem Ertürk, Bo Mattiasson
Nov 6, 2018·ACS Applied Materials & Interfaces·Mahalia A C SerranoRichard W Vachet

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