Dye-ligand and metal chelate poly(2-hydroxyethylmethacrylate) membranes for affinity separation of proteins

Journal of Chromatography. a
M Yakup AricaA Denizli

Abstract

Cibacron Blue F3GA was covalently immobilized onto poly(2-hydroxyethyl methacrylate) pHEMA) membranes via the nucleophilic reaction between the chloride of its triazine ring and the hydroxyl group of pHEMA. Then, Fe3+ ions were complexed by chelation with the immobilized Cibacron Blue F3GA molecules. Different amounts of Fe3+ ions were loaded on the membranes by changing the concentration of Fe3+ ions and pH of the reaction medium. Membranes with or without Fe3+ were used in the adsorption of glucose oxidase, catalase and bovine serum albumin. The adsorption capacities of these membranes were determined by changing pH and the concentration of the proteins in the adsorption medium. The adsorption phenomena appeared to follow a typical Langmuir isotherm. The maximum capacities (qm) of the Fe3+ complexed membranes for glucose oxidase, catalase and bovine serum albumin (8.70 x 10(-3) mumol m-2, 2.15 x 10(-3) mumol m-2 and 2.21 x 10(-3) mumol m-2) were greater than those of the untreated membranes (6.79 x 10(-3) mumol m-2, 1.34 x 10(-3) mumol m-2 and 1.94 x 10(-3) mumol m-2) respectively. The nonspecific adsorption of the enzymes and the protein on the pHEMA membranes was negligible.

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Citations

Nov 6, 2001·Journal of Biochemical and Biophysical Methods·V Gaberc-Porekar, V Menart
Nov 6, 2001·Journal of Biochemical and Biophysical Methods·A Denizli, E Pişkin
Feb 1, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yueh-Hua TsaiShing-Yi Suen
Nov 25, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Shing-Yi SuenChao-Shuan Chang
Sep 6, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Adil DenizliErhan Pişkin
Mar 30, 2005·Journal of Biomaterials Science. Polymer Edition·Nursel PekelOlgun Güven
Mar 24, 2010·Colloids and Surfaces. B, Biointerfaces·Juan M BolivarJóse M Guisán
Mar 24, 2016·Artificial Cells, Nanomedicine, and Biotechnology·Müşerref CakaAdil Denizli
Jun 19, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·M Yakup AricaGülay Bayramoğlu
Feb 7, 2004·Biotechnology Progress·Sibel EmirAdil Denizli
Jul 9, 2005·Journal of Biomaterials Science. Polymer Edition·Handan YavuzAdil Denizli
Aug 5, 2005·Macromolecular Bioscience·Sinan AkgölAdil Denizli
Jan 31, 2012·Journal of Structural and Functional Genomics·Heungbok KimChang-Yub Kim

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