Dynactin and Num1 cooperate to establish the cortical anchoring of cytoplasmic dynein in S. pombe

Journal of Cell Science
Ikumi FujitaMasayuki Yamamoto

Abstract

Chromosome movement during meiosis is crucial for homologous pairing and meiotic recombination. During meiotic prophase in fission yeast, rapid nuclear migration is dependent on cytoplasmic dynein, which is anchored to the cell cortex and pulls microtubules, thereby driving nuclear migration. However, the precise mechanisms underlying dynein localization and activation remain unclear. Here, we identified three subunits of dynactin in fission yeast: Arp1, Mug5 and Jnm1 (also known as Mug1). These subunits transiently colocalized with dynein foci at the cell cortex and were essential for the cortical anchoring of dynein. Cortical factor Num1 (also known as Mcp5), which was also required for dynein anchoring, bound to dynein independently of dynactin. Whereas Num1 suppressed the sliding of dynein foci along the cortex, Arp1, Mug5 and Jnm1 were involved in the regulation of shrinkage and bundling of microtubules. From these data, we propose that dynein anchoring is established by cooperation of transient assembly of dynactin and function of Num1 at the cell cortex.

References

Apr 8, 1994·Science·Y ChikashigeY Hiraoka
Jan 6, 2000·Nature Cell Biology·S J King, T A Schroer
Aug 2, 2000·The Journal of Biological Chemistry·S KumarM Plamann
Jul 20, 2001·The Journal of Biological Chemistry·E A HolleranE L Holzbaur
Feb 5, 2003·The Journal of Cell Biology·Wei-Lih LeeJohn A Cooper
Apr 18, 2003·Nature·Joe Howard, Anthony A Hyman
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Trina A Schroer
Oct 29, 2004·Journal of Cell Science·Teresa NiccoliMasayuki Yamamoto
Jan 12, 2005·The Journal of Cell Biology·Wei-Lih LeeJohn A Cooper
Jan 22, 2005·Current Opinion in Cell Biology·Anna Akhmanova, Casper C Hoogenraad
May 11, 2005·Neuron·Li-Huei Tsai, Joseph G Gleeson
Aug 23, 2005·Current Biology : CB·Kayoko TanakaMasayuki Yamamoto
Nov 24, 2005·Current Biology : CB·Cristina Martín-CastellanosSergio Moreno
Feb 14, 2006·Nature Cell Biology·Tara L Culver-HanlonStephen J King
Jul 11, 2006·Nature·Yuriko HarigayaMasayuki Yamamoto
Aug 16, 2006·Biology of the Cell·Jean-Baptiste Manneville, Sandrine Etienne-Manneville
Apr 16, 2008·Journal of Cell Science·Fabrice CaudronCécile Boscheron
May 17, 2011·Developmental Cell·Steven M Markus, Wei-Lih Lee
Mar 27, 2012·Current Biology : CB·Adam G HendricksErika L F Holzbaur
Feb 13, 2013·The Journal of Cell Biology·Masashi YoshidaAyumu Yamamoto
Jun 26, 2013·Cell·Vaishnavi AnanthanarayananIva M Tolić-Nørrelykke

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Citations

May 5, 2016·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Vaishnavi Ananthanarayanan
Mar 16, 2017·Proceedings of the National Academy of Sciences of the United States of America·Jerrin Mathew ThankachanVaishnavi Ananthanarayanan
Jul 25, 2017·Cold Spring Harbor Protocols·Akira YamashitaMasayuki Yamamoto
Oct 8, 2020·Cell Reports·Johnathan L MeadersDavid R Burgess

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