Dynamic and Assembly of Epiphyte and Endophyte Lactic Acid Bacteria During the Life Cycle of Origanum vulgare L

Frontiers in Microbiology
Erica PontonioMarco Gobbetti

Abstract

Origanum vulgare L. (oregano) was chosen as suitable model to investigate the ability of the endophyte-microbiome, especially that of lactic acid bacteria, to develop specific interactions with the plant, mediated by the essential oils (EOs). Combined culture-dependent and -independent approaches analyzed the bacterial dynamic and assembly of Origanum vulgare L. throughout the life cycle. Epiphyte bacteria were more abundant than the endophyte ones. The number of presumptive lactic acid bacteria increased throughout oregano life cycle, according to the plant organ. Diverse species of lactic acid bacteria populated the plant, but Lactobacillus plantarum stably dominated both epiphyte and endophyte populations. High-throughput DNA sequencing showed highest epiphyte bacterial diversity at early vegetative and full-flowering stages, with blooming signing the main microbial differentiation among plant organs. Proteobacteria, Actinobacteria and Bacteroidetes, and Firmicutes and Cyanobacteria at lower abundance were the main phyla. Various genera were detectable, but oregano harbored mainly Methylobacterium, Sphingomonas, Rhizobium and Aurantimonas throughout phenological stages. Firmicutes epiphyte and endophyte microbiotas were diff...Continue Reading

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Citations

Oct 2, 2019·Environmental Microbiology Reports·Annabelle O YuMaria L Marco
Oct 18, 2019·Frontiers in Microbiology·Laura SantamaríaFélix López de Felipe
Aug 8, 2020·Frontiers in Microbiology·Marie LegeinSarah Lebeer
May 1, 2020·Frontiers in Microbiology·Mauricio Edilberto Pacheco-MontealegreAlejandro Caro-Quintero
Jul 30, 2021·Frontiers in Microbiology·Ashraf Al AshhabGidon Winters
Aug 7, 2021·Frontiers in Microbiology·Simon SauerMeinhard Simon
Sep 24, 2021·Microbial Ecology·Loan Edel Villalobos-FloresJaime García-Mena
Jan 22, 2022·International Journal of Systematic and Evolutionary Microbiology·Marie LegeinSarah Lebeer

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Datasets Mentioned

BETA
PRJNA347573
EZ-TAXON
KT626386.1
KJ187149.1
KJ187148.1
KJ187133.1
JN851776.1
AB598965.1
KJ187143.1

Methods Mentioned

BETA
biotyping
PCR
electrophoresis

Software Mentioned

USEARCH
Python
QIIME
Statistica
PermutMatrix
EZ
UBLAST

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