Dynamic and complex Mycobacterium tuberculosis microevolution unrevealed by standard genotyping

Tuberculosis
Laura Pérez-LagoDarío García de Viedma

Abstract

We performed an in-depth analysis of a large Mycobacterium tuberculosis cluster involving nine isolates with identical or highly similar RFLP types. The strain involved in this transmission cluster microevolved and accumulated up to five differential IS6110 transposition events, several of them not revealed by standard genotyping approaches. Some of these events probably generated extended deletions. In two cases of tuberculosis within the cluster, we observed the simultaneous presence of both the reference and microevolved variants. A longitudinal genotypical survey of M. tuberculosis in a real clinical evolutionary scenario can reveal dynamic and complex microevolution events.

References

Nov 28, 2002·Emerging Infectious Diseases·Kashef IjazM Donald Cave
Feb 10, 2004·Journal of Clinical Microbiology·S L SampsonR M Warren
Apr 29, 2006·The Pediatric Infectious Disease Journal·Darío García de ViedmaEmilio Bouza
Feb 5, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·L TurciosL Salazar
Jul 2, 2010·Journal of Clinical Microbiology·Anita C SchürchDick van Soolingen
Aug 6, 2010·Journal of Clinical Microbiology·Sahal A M Al-HajojAdri G M van der Zanden
Sep 24, 2011·Journal of Clinical Microbiology·Laura Pérez-LagoDarío García de Viedma

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Citations

Apr 4, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Adrian MuwongeTone B Johansen
Mar 19, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Laura Pérez-LagoDarío García-de-Viedma

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