Dynamic and scalable DNA-based information storage.

Nature Communications
Kevin LinAlbert J Keung

Abstract

The physical architectures of information storage systems often dictate how information is encoded, databases are organized, and files are accessed. Here we show that a simple architecture comprised of a T7 promoter and a single-stranded overhang domain (ss-dsDNA), can unlock dynamic DNA-based information storage with powerful capabilities and advantages. The overhang provides a physical address for accessing specific DNA strands as well as implementing a range of in-storage file operations. It increases theoretical storage densities and capacities by expanding the encodable sequence space and simplifies the computational burden in designing sets of orthogonal file addresses. Meanwhile, the T7 promoter enables repeatable information access by transcribing information from DNA without destroying it. Furthermore, saturation mutagenesis around the T7 promoter and systematic analyses of environmental conditions reveal design criteria that can be used to optimize information access. This simple but powerful ss-dsDNA architecture lays the foundation for information storage with versatile capabilities.

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Citations

Jan 19, 2021·Trends in Biotechnology·Cheng Kai LimChueh Loo Poh
Apr 10, 2021·Nucleic Acids Research·Chengtao XuHong Liu
May 22, 2021·Frontiers in Genetics·Yanfen ZhengBin Wang
Jun 12, 2021·Nature Communications·Kyle J TomekAlbert J Keung
Jun 12, 2021·Nature Materials·James L BanalMark Bathe
Oct 2, 2020·Biotechnology Advances·Philip M StanleyKevin C K Lee
Aug 27, 2021·Nature Materials·Luca Piantanida, William L Hughes

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Methods Mentioned

BETA
PCR
electrophoresis
in vitro transcription
RNA
Fluorescence imaging
common

Software Mentioned

DORIS
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FIJI
Oligonucleotide Properties Calculator

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