Accurate positioning of the division septum at the equator of Escherichia coli cells requires a rapid oscillation of MinD ATPase between the polar halves of the cell membrane, together with the division inhibitor MinC, under MinE control. The mechanism underlying MinD oscillation remains poorly understood. Here, we demonstrate that purified MinD assembles into protein filaments in the presence of ATP. Incubation with phospholipid vesicles further stimulates MinD polymerization. Addition of purified MinE in the presence of lipids promotes bundling of MinD filaments as well as their disassembly through activation of MinD ATPase. MinE thus provokes a net decay in the steady-state MinD polymer mass. Taken together, our results suggest that reversible MinD assembly modulated by MinE underlies the dynamic processing of positional information in E. coli to identify precisely the nascent site for cell division.
Central role for the Escherichia coli minC gene product in two different cell division-inhibition systems
A division inhibitor and a topological specificity factor coded for by the minicell locus determine proper placement of the division septum in E. coli
Proper placement of the Escherichia coli division site requires two functions that are associated with different domains of the MinE protein
Cloning of a human cDNA encoding a putative nucleotide-binding protein related to Escherichia coli MinD
Deletion analysis of gene minE which encodes the topological specificity factor of cell division in Escherichia coli
FtsH (HflB) is an ATP-dependent protease selectively acting on SecY and some other membrane proteins
The MinE ring: an FtsZ-independent cell structure required for selection of the correct division site in E. coli
The relationship between hetero-oligomer formation and function of the topological specificity domain of the Escherichia coli MinE protein
Rapid pole-to-pole oscillation of a protein required for directing division to the middle of Escherichia coli
FtsZ ring clusters in min and partition mutants: role of both the Min system and the nucleoid in regulating FtsZ ring localization
Selection of the midcell division site in Bacillus subtilis through MinD-dependent polar localization and activation of MinC
Topological regulation of cell division in Escherichia coli involves rapid pole to pole oscillation of the division inhibitor MinC under the control of MinD and MinE
The MinC component of the division site selection system in Escherichia coli interacts with FtsZ to prevent polymerization
Dynamic movement of the ParA-like Soj protein of B. subtilis and its dual role in nucleoid organization and developmental regulation
A homologue of the bacterial cell division site-determining factor MinD mediates placement of the chloroplast division apparatus
Structural and functional studies of MinD ATPase: implications for the molecular recognition of the bacterial cell division apparatus
Topological regulation of cell division in E. coli. spatiotemporal oscillation of MinD requires stimulation of its ATPase by MinE and phospholipid
The double par locus of virulence factor pB171: DNA segregation is correlated with oscillation of ParA
Division site placement in E.coli: mutations that prevent formation of the MinE ring lead to loss of the normal midcell arrest of growth of polar MinD membrane domains
RNaseE and RNA helicase B play central roles in the cytoskeletal organization of the RNA degradosome
Streptococcus pyogenes pSM19035 requires dynamic assembly of ATP-bound ParA and ParB on parS DNA during plasmid segregation
ATP-regulated interactions between P1 ParA, ParB and non-specific DNA that are stabilized by the plasmid partition site, parS
Bacillus subtilis MinC destabilizes FtsZ-rings at new cell poles and contributes to the timing of cell division
Self-assembly of the bacterial cytoskeleton-associated RNA helicase B protein into polymeric filamentous structures
Eicosapentaenoic acid plays a beneficial role in membrane organization and cell division of a cold-adapted bacterium, Shewanella livingstonensis Ac10
Membrane binding by MinD involves insertion of hydrophobic residues within the C-terminal amphipathic helix into the bilayer
Mapping the MinE site involved in interaction with the MinD division site selection protein of Escherichia coli
The N terminus of MinD contains determinants which affect its dynamic localization and enzymatic activity
ZipA is required for targeting of DMinC/DicB, but not DMinC/MinD, complexes to septal ring assemblies in Escherichia coli
Analysis of MinD mutations reveals residues required for MinE stimulation of the MinD ATPase and residues required for MinC interaction
Phosphatidylethanolamine domains and localization of phospholipid synthases in Bacillus subtilis membranes
Towards the development of Bacillus subtilis as a cell factory for membrane proteins and protein complexes
Fluorescence polarization analysis for revealing molecular mechanism of nucleotide-dependent phospholipid membrane binding of MinD adenosine 5'-triphosphate, adenosine triphosphatase
Subcellular Min oscillations as a single-cell reporter of the action of polycations, protamine, and gentamicin on Escherichia coli
A man-made ATP-binding protein evolved independent of nature causes abnormal growth in bacterial cells
The N-terminal amphipathic helix of the topological specificity factor MinE is associated with shaping membrane curvature
A polymerization-depolymerization model that accurately generates the self-sustained oscillatory system involved in bacterial division site placement
The tail of the ParG DNA segregation protein remodels ParF polymers and enhances ATP hydrolysis via an arginine finger-like motif
Division site selection in Escherichia coli involves dynamic redistribution of Min proteins within coiled structures that extend between the two cell poles
RNaseE and the other constituents of the RNA degradosome are components of the bacterial cytoskeleton
Positioning of the MinE binding site on the MinD surface suggests a plausible mechanism for activation of the Escherichia coli MinD ATPase during division site selection
Screening for synthetic lethal mutants in Escherichia coli and identification of EnvC (YibP) as a periplasmic septal ring factor with murein hydrolase activity
Regular cellular distribution of plasmids by oscillating and filament-forming ParA ATPase of plasmid pB171
A conserved polar region in the cell division site determinant MinD is required for responding to MinE-induced oscillation but not for localization within coiled arrays
Mapping of the interactions between partition proteins Delta and Omega of plasmid pSM19035 from Streptococcus pyogenes
Molecular Interactions of the Min Protein System Reproduce Spatiotemporal Patterning in Growing and Dividing Escherichia coli Cells
An Optimal Free Energy Dissipation Strategy of the MinCDE Oscillator in Regulating Symmetric Bacterial Cell Division
A hypothesis to explain division site selection in Escherichia coli by combining nucleoid occlusion and Min
The Min-protein oscillations in Escherichia coli : an example of self-organized cellular protein waves
Dissecting the role of conformational change and membrane binding by the bacterial cell division regulator MinE in the stimulation of MinD ATPase activity
A stochastic model of Min oscillations in Escherichia coli and Min protein segregation during cell division
Self-assembly of MinE on the membrane underlies formation of the MinE ring to sustain function of the Escherichia coli Min system
Min-protein oscillations in Escherichia coli with spontaneous formation of two-stranded filaments in a three-dimensional stochastic reaction-diffusion model
Reconstitution of pole-to-pole oscillations of min proteins in microengineered polydimethylsiloxane compartments
Septal membrane localization by C-terminal amphipathic α-helices of MinD in Bacillus subtilis mutant cells lacking MinJ or DivIVA
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