Dynamic association between alpha-actinin and beta-integrin regulates contraction of canine tracheal smooth muscle

The Journal of Physiology
Wenwu Zhang, S J Gunst

Abstract

The adhesion junctions of smooth muscle cells may be dynamically regulated during smooth muscle contraction, and this dynamic regulation may be important for the development of active tension. In the present study, the role of alpha-actinin during smooth muscle contraction was evaluated in tracheal smooth muscle tissues and freshly dissociated cells. Stimulation with acetylcholine (ACh) increased the localization of alpha-actinin at the membrane of freshly dissociated smooth muscle cells, and increased the amount of beta1 integrin that coprecipitated with alpha-actinin from muscle tissue homogenates. GFP-alpha-actinin fusion proteins were expressed in muscle tissues and visualized in live freshly dissociated cells. GFP-alpha-actinin translocated to the membrane within seconds of stimulation of the cells with ACh. Expression of the integrin-binding rod domain of alpha-actinin in smooth muscle tissues depressed active contraction in response to ACh. Expression of the alpha-actinin rod domain also inhibited the translocation of endogenous alpha-actinin to the membrane, and inhibited the association of endogenous alpha-actinin with beta1-integrin in alpha-actinin immunoprecipitates from tissue extracts. However, the expression of a...Continue Reading

References

Aug 1, 1990·The Journal of Cell Biology·C A OteyK Burridge
Sep 15, 1989·Biochemical and Biophysical Research Communications·J Nishimura, C van Breemen
Jan 1, 1989·Annual Review of Physiology·K E Kamm, J T Stull
Mar 1, 1983·The Journal of Cell Biology·F S FayK E Fogarty
Jan 1, 1983·Cell Motility·K B AdlerJ N Evans
May 1, 1995·The American Journal of Physiology·S J GunstM Rowe
Apr 1, 1996·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·S Y SaitoH Karaki
Jan 1, 1996·Annual Review of Cell and Developmental Biology·K Burridge, M Chrzanowska-Wodnicka
Feb 1, 1997·Current Opinion in Cell Biology·K M Yamada, B Geiger
Jun 8, 2001·Journal of Muscle Research and Cell Motility·M E FultzG L Wright
Oct 17, 2001·The Journal of Biological Chemistry·M BárányK Bárány
Jan 5, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Marilyn J CipollaGeorge Osol
Jul 18, 2002·The Journal of Physiology·Dale D TangSusan J Gunst
Aug 15, 2002·American Journal of Physiology. Cell Physiology·Steven S AnJeffrey J Fredberg
Apr 30, 2003·The Journal of Biological Chemistry·Tamara S FraleyJeffrey A Greenwood
Jun 10, 2003·Journal of Applied Physiology·Susan J Gunst, Jeffrey J Fredberg
Oct 1, 2003·Respiratory Physiology & Neurobiology·Susan J GunstAnabelle Opazo Saez
Oct 23, 2003·Annual Review of Cell and Developmental Biology·Alexander D BershadskyBenjamin Geiger
Oct 25, 2003·American Journal of Physiology. Cell Physiology·Anabelle Opazo SaezSusan J Gunst
Dec 13, 2003·The Biochemical Journal·Anne Marie CorganJeffrey A Greenwood
Jan 31, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ana M HerreraChun Y Seow
Apr 15, 2004·Cell Motility and the Cytoskeleton·Carol A Otey, Olli Carpen
May 11, 2004·Molecular Biology of the Cell·Lynda J PetersonKeith Burridge
Sep 25, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Nicholas A FlavahanSheila Flavahan
Dec 31, 2004·American Journal of Physiology. Cell Physiology·Wenwu ZhangSusan J Gunst
Feb 16, 2005·The Journal of Biological Chemistry·Tamara S FraleyJeffrey A Greenwood
Feb 22, 2005·Journal of Structural Biology·Deborah F Kelly, Kenneth A Taylor
Jul 22, 2005·American Journal of Physiology. Cell Physiology·Thomas J EddingerDarl R Swartz

❮ Previous
Next ❯

Citations

Nov 13, 2010·The Journal of Biological Chemistry·Youliang HuangSusan J Gunst
Nov 26, 2008·The Journal of Biological Chemistry·Mikael K SchnizlerMichael J Welsh
Sep 8, 2007·Canadian Journal of Physiology and Pharmacology·Chun Y Seow, Peter D Paré
Feb 8, 2008·American Journal of Physiology. Cell Physiology·Dale D Tang
Jul 4, 2008·American Journal of Physiology. Cell Physiology·Susan J Gunst, Wenwu Zhang
Sep 23, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Yidi WuSusan J Gunst
Dec 4, 2010·Journal of Applied Physiology·Chun Y Seow, Jeffrey J Fredberg
May 14, 2009·PLoS Computational Biology·Javad GoljiMohammad R K Mofrad
Dec 21, 2007·Proceedings of the American Thoracic Society·Wenwu Zhang, Susan J Gunst
Oct 16, 2012·Trends in Molecular Medicine·Ynuk Bossé
Dec 23, 2014·Canadian Journal of Physiology and Pharmacology·Wenwu ZhangSusan J Gunst
Jan 24, 2015·Canadian Journal of Physiology and Pharmacology·Bo LanLinhong Deng
Dec 6, 2016·The Journal of Clinical Investigation·Aparna SundaramDean Sheppard
Oct 18, 2012·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Michael P Walsh, William C Cole
Jun 10, 2017·Journal of Cell Science·Pasquale ChitanoChun Y Seow
Jul 2, 2014·Integrative Biology : Quantitative Biosciences From Nano to Macro·Harini SreenivasappaAndreea Trache
Sep 22, 2006·American Journal of Physiology. Cell Physiology·Elaine M SmolockRobert S Moreland
Nov 4, 2006·The Journal of Biological Chemistry·Savita V DandapaniMartin R Pollak
May 15, 2007·Journal of Applied Physiology·Farah AliChun Y Seow
May 18, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Sepp R JansenReinoud Gosens
May 25, 2013·American Journal of Physiology. Cell Physiology·Ransom H PoythressKathleen G Morgan
Mar 5, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Youliang Huang, Susan J Gunst
Jun 11, 2020·PLoS Computational Biology·Xiaona LiYi Jiang
Sep 27, 2018·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Jacob M Jaslove, Celeste M Nelson
Feb 1, 2019·Journal of Engineering and Science in Medical Diagnostics and Therapy·Wenwu Zhang, Susan J Gunst
Jan 13, 2015·Journal of Applied Physiology·Audrey Lee-GosselinYnuk Bossé
Mar 21, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Randolph L CortelingDonald G Welsh
May 15, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Klodiana Jani, Frieder Schöck
May 20, 2021·American Journal of Physiology. Lung Cellular and Molecular Physiology·Yidi WuSusan J Gunst
Jun 15, 2021·Frontiers in Molecular Biosciences·Patricia FranzkaChristian A Hübner
Aug 4, 2021·Advanced Healthcare Materials·Cara T MotzCheng Zhu
May 5, 2009·Pulmonary Pharmacology & Therapeutics·Linhong DengNewman L Stephen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.