Dynamic bioluminescence imaging for quantitative tumour burden assessment using IV or IP administration of D: -luciferin: effect on intensity, time kinetics and repeatability of photon emission

European Journal of Nuclear Medicine and Molecular Imaging
Marleen KeyaertsTony Lahoutte

Abstract

In vivo bioluminescence imaging (BLI) is a promising technique for non-invasive tumour imaging. D: -luciferin can be administrated intraperitonealy or intravenously. This will influence its availability and, therefore, the bioluminescent signal. The aim of this study is to compare the repeatability of BLI measurement after IV versus IP administration of D: -luciferin and assess the correlation between photon emission and histological cell count both in vitro and in vivo. Fluc-positive R1M cells were subcutaneously inoculated in nu/nu mice. Dynamic BLI was performed after IV or IP administration of D: -luciferin. Maximal photon emission (PE(max)) was calculated. For repeatability assessment, every acquisition was repeated after 4 h and analysed using Bland-Altman method. A second group of animals was serially imaged, alternating IV and IP administration up to 21 days. When mice were killed, PE(max) after IV administration was correlated with histological cell number. The coefficients of repeatability were 80.2% (IV) versus 95.0% (IP). Time-to-peak is shorter, and its variance lower for IV (p < 0.0001). PE(max) was 5.6 times higher for IV. A trend was observed towards lower photon emission per cell in larger tumours. IV administr...Continue Reading

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Citations

Dec 20, 2008·European Journal of Nuclear Medicine and Molecular Imaging·Yusuke InoueKuni Ohtomo
Nov 26, 2009·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Laura MezzanotteAldo Roda
Apr 13, 2010·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Marleen KeyaertsTony Lahoutte
Jan 31, 2013·Nature Communications·Hidekazu SekineTeruo Okano
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Jun 8, 2012·Endocrine-related Cancer·Barbara MarinielloFranco Mantero
Jun 3, 2014·Theranostics·Matthias D'HuyvetterTony Lahoutte
Sep 25, 2010·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Lea Olive Tchouate GainkamTony Lahoutte
Feb 11, 2012·Trends in Molecular Medicine·Marleen KeyaertsTony Lahoutte
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Jun 23, 2015·Oncoscience·Isabella Monia MontagnerAntonio Rosato
Feb 13, 2014·Oncotarget·Cleo GoyvaertsKarine Breckpot

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