Dynamic Change and Target Prediction of Axon-Specific MicroRNAs in Regenerating Sciatic Nerve

PloS One
Monichan PhaySoonmoon Yoo

Abstract

Injury to axons in the peripheral nervous system induces rapid and local regenerative responses to form a new growth cone, and to generate a retrogradely transporting injury signal. The evidence for essential roles of intra-axonal protein synthesis during regeneration is now compelling. MicroRNA (miRNA) has recently been recognized as a prominent player in post-transcriptional regulation of axonal protein synthesis. Here, we directly contrast temporal changes of miRNA levels in the sciatic nerve following injury, as compared to those in an uninjured nerve using deep sequencing. Small RNAs (<200 nucleotides in length) were fractionated from the proximal nerve stumps to improve the representation of differential miRNA levels. Of 141 axoplasmic miRNAs annotated, 63 rat miRNAs showed significantly differential levels at five time points following injury, compared to an uninjured nerve. The differential changes in miRNA levels responding to injury were processed for hierarchical clustering analyses, and used to predict target mRNAs by Targetscan and miRanda. By overlapping these predicted targets with 2,924 axonally localizing transcripts previously reported, the overlapping set of 214 transcripts was further analyzed by the Gene On...Continue Reading

References

Dec 23, 2003·Neuron·Shlomit HanzMike Fainzilber
Jan 14, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Poonam VermaJames W Fawcett
Aug 11, 2006·Journal of Neurochemistry·Shlomit Hanz, Mike Fainzilber
Nov 23, 2006·Nature Reviews. Neuroscience·Kenneth S Kosik
Jan 9, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Alexander K MurashovMichael R Van Scott
Jan 31, 2008·RNA·Mads BakSakari Kauppinen
Feb 20, 2009·Journal of Neuropathology and Experimental Neurology·Cory C TothDouglas W Zochodne
Mar 20, 2009·Human Molecular Genetics·Marc S Weinberg, Matthew J A Wood
Jun 13, 2009·Experimental Neurology·Laura F GumyJames W Fawcett
Jul 7, 2009·Experimental Neurology·Nai-Kui LiuXiao-Ming Xu
Nov 3, 2009·Current Genomics·Olivier C MaesHyman M Schipper
Nov 4, 2009·Developmental Neurobiology·Ida RishalMike Fainzilber
Oct 26, 2010·Journal of Visualized Experiments : JoVE·Ida RishalMike Fainzilber
Mar 10, 2011·Physiological Genomics·Nai-Kui Liu, Xiao-Ming Xu
Oct 4, 2011·The EMBO Journal·Christopher J DonnellyJeffery L Twiss
Oct 14, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Dianna E WillisJeffery L Twiss
Jan 17, 2012·The EMBO Journal·Keren Ben-YaakovMike Fainzilber
Jul 31, 2012·Neuron·Rotem Ben-Tov PerryMike Fainzilber
Oct 26, 2012·Journal of Cell Science·Tanuja T MeriandaJeffery L Twiss
Aug 24, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Tanuja T MeriandaJeffery L Twiss
Dec 11, 2013·Frontiers in Molecular Neuroscience·Sharof A KhudayberdievGerhard Schratt

❮ Previous
Next ❯

Citations

Apr 28, 2016·Brain Research·Vicente ValenzuelaFelipe A Court
Jun 9, 2017·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Amar N KarJeffery L Twiss
Dec 15, 2016·Molecular Neurobiology·Monichan PhaySoonmoon Yoo
Dec 10, 2016·Journal of Molecular Cell Biology·Bin Wang, Lan Bao
Jun 16, 2017·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Jenna R GaleBarry B Kaplan
Apr 17, 2019·Experimental and Therapeutic Medicine·Chu ChenTianmei Qian
Oct 1, 2016·Applied Biochemistry and Biotechnology·Zeinab AhsaniMahdi Paryan
Apr 15, 2018·Journal of Cell Science·Pabitra K SahooJeffery L Twiss
Mar 23, 2018·Frontiers in Molecular Neuroscience·Jian WengBaoguo Jiang
Jan 22, 2020·Neural Regeneration Research·Jian WengXiao-Feng Yin
Nov 22, 2020·International Journal of Molecular Sciences·Eloina Corradi, Marie-Laure Baudet
May 4, 2020·Brain Research·Terika P SmithJeffery L Twiss
Jul 13, 2021·Frontiers in Cellular Neuroscience·Andres Di PaoloJosé Roberto Sotelo-Silveira

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
Genotyping

Software Mentioned

piRNABank
TreeView
miRanda
TargetScan
CLC Genomics Workbench ( Qiagen )
Ingenuity Pathway Analysis ( IPA )
edgeR
Cluster

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.