Dynamic changes in connexin expression following engraftment of neural stem cells to striatal tissue

Experimental Cell Research
Johan JäderstadEric Herlenius

Abstract

Gap-junctional intercellular communication between grafted neural stem cells (NSCs) and host cells seem to be essential for many of the beneficial effects associated with NSC engraftment. Utilizing murine NSCs (mNSCs) grafted into an organotypic ex vivo model system for striatal tissue we examined the prerequisites for formation of gap-junctional couplings between graft and host cells at different time points following implantation. We utilized flow cytometry (to quantify the proportion of connexin (Cx) 26 and 43 expressing cells), immunohistochemistry (for localization of the gap-junctional proteins in graft and host cells), dye-transfer studies with and without pharmacological gap-junctional blockers (assaying the functionality of the formed gap-junctional couplings), and proliferation assays (to estimate the role of gap junctions for NSC well-being) to this end. Immunohistochemical staining and dye-transfer studies revealed that the NSCs already form functional gap junctions prior to engraftment, thereby creating a substrate for subsequent graft and host communication. The expression of Cx43 by grafted NSCs was decreased by neurotrophin-3 overexpression in NSCs and culturing of grafted tissue in serum-free Neurobasal B27 med...Continue Reading

Citations

Jul 14, 2012·Stem Cells International·Anna JezierskiMahmud Bani-Yaghoub
Dec 7, 2013·Neuropathology and Applied Neurobiology·G Cisbani, F Cicchetti
Mar 21, 2012·Archives of Biochemistry and Biophysics·Rekha KarJean X Jiang
Aug 1, 2013·Experimental Neurology·Nicolas DaviaudClaudia N Montero-Menei
Nov 17, 2012·Current Protocols in Stem Cell Biology·Eric HerleniusPetri Olivius
Aug 15, 2017·Current Protocols in Stem Cell Biology·David ForsbergEric Herlenius
Jun 27, 2020·Neurological Research·Hiroaki KenmochiHiroki Namba

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