Dynamic Changes in Equatorial Segment Protein 1 (SPESP1) Glycosylation During Mouse Spermiogenesis

Biology of Reproduction
Viswanadhapalli SuryavathiJohn C Herr

Abstract

ESP1/SPESP1 is a testis-specific, postmeiotic gene expressed in round spermatids that encodes equatorial segment protein 1, an intra-acrosomal protein found in the acrosomal matrix and on the luminal surface of the inner and outer acrosomal membranes within the equatorial segment domain of mature spermatozoa. A comparison of testicular protein extracts with caput, corpus, and caudal epididymal sperm proteins revealed striking differences in the apparent masses of SPESP1 isoforms. The predominant isoforms of SPESP1 in the testis were 77 and 67 kDa, with 47-kDa forms present to a minor degree. In contrast, SPESP1 isoforms of 47 and 43 kDa were found in caput, corpus, and caudal sperm, indicating that SPESP1 undergoes noticeable mass changes during spermiogenesis and/or subsequent transport to the epididymis. On two-dimensional (2D) SDS-PAGE, testicular SPESP1 isoforms resolved as a train of pI values from 4.9 to 5.2. Immunoprecipitated 77-kDa SPESP1 from testis reacted with the glycoprofile stain after one-dimensional and 2D gel electrophoresis, indicating that the 77-kDa testicular isoform was highly glycosylated. One charge variant of the 67-kDa isoform was also glycoprofile positive after 2D gel resolution. The 47- and 43-kDa ...Continue Reading

References

Nov 1, 1979·Journal of Biochemistry·Y UchidaT Sugimori
Feb 1, 1977·Biology of Reproduction·D M Phillips
Mar 1, 1979·Experimental Cell Research·J M BedfordL E Franklin
Jul 1, 1991·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J M FeinbergS J Weinman
Dec 1, 1988·The Journal of Experimental Zoology·R Yanagimachi, A Bhattacharyya
Aug 13, 1985·Biochemistry·A L TarentinoT H Plummer
Apr 1, 1970·Journal of Reproduction and Fertility·A C Allison, E F Hartree
Nov 1, 1974·The Journal of Cell Biology·D S Friend, D W Fawcett
Sep 26, 2001·Reproduction : the Official Journal of the Society for the Study of Fertility·K YoshinagaK Toshimori
Oct 2, 2004·Andrologia·M KamaruddinW A King
Jun 29, 2005·Developmental Biology·María Belén HerreroJohn C Herr
Jan 18, 2006·Methods in Enzymology·Jasna Peter-Katalinić
Jan 11, 2007·Journal of Mass Spectrometry : JMS·Jamshid KhoshnoodiDavid B Friedman
Jun 28, 2007·Molecular Human Reproduction·L A MitchellR J Aitken
Jun 16, 2009·Journal of Proteome Research·Stephen A WhelanHelena R Chang
Jul 17, 2009·Biology of Reproduction·Kenji YamatoyaKiyotaka Toshimori
Nov 10, 2009·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Zheng-mei LvChen Xu
Apr 9, 2010·Journal of Cell Science·Yoshitaka FujiharaMasaru Okabe

❮ Previous
Next ❯

Citations

Jan 23, 2016·Journal of Proteome Research·Mayank SaraswatRisto Renkonen
Jan 18, 2016·Seminars in Cell & Developmental Biology·Giovanna Berruti
Sep 13, 2015·Reproductive Sciences·Min Liu
Feb 5, 2019·Reproduction, Fertility, and Development·Peng ZhangLiqing Fan
Jan 11, 2016·Anatomical Science International·Chizuru Ito, Kiyotaka Toshimori
Mar 17, 2018·Frontiers in Cellular and Infection Microbiology·Lei ZhangJinhai Huang
Oct 18, 2019·Frontiers in Cell and Developmental Biology·Muhammad Babar KhawarWei Li
Jan 27, 2019·Scientific Reports·Cristina Pérez-PatiñoInmaculada Parrilla
Feb 11, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Liza O'DonnellPeter G Stanton

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
cDNA
immunoprecipitation
glycosylation
deamidation

Software Mentioned

Expasy glycosylation
Expasy
Basic Local Alignment Search Tool ( BLAST )
NetOGlyc

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.