Dynamic changes in lung responses after single and repeated exposures to cigarette smoke in mice

PloS One
Michelle L EngleClaire M Doerschuk

Abstract

Cigarette smoke is well recognized to cause injury to the airways and the alveolar walls over time. This injury usually requires many years of exposure, suggesting that the lungs may rapidly develop responses that initially protect it from this repetitive injury. Our studies tested the hypotheses that smoke induces an inflammatory response and changes in mRNA profiles that are dependent on sex and the health status of the lung, and that the response of the lungs to smoke differs after 1 day compared to 5 days of exposure. Male and female wildtype (WT) and Scnn1b-transgenic (βENaC) mice, which have chronic bronchitis and emphysematous changes due to dehydrated mucus, were exposed to cigarette smoke or sham air conditions for 1 or 5 days. The inflammatory response and gene expression profiles were analyzed in lung tissue. Overall, the inflammatory response to cigarette smoke was mild, and changes in mediators were more numerous after 1 than 5 days. βENaC mice had more airspace leukocytes than WT mice, and smoke exposure resulted in additional significant alterations. Many genes and gene sets responded similarly at 1 and 5 days: genes involved in oxidative stress responses were upregulated while immune response genes were downregu...Continue Reading

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Datasets Mentioned

BETA
GSE109776

Methods Mentioned

BETA
bronchoalveolar lavage
PCR
light microscopy
ELISA
flow cytometry

Software Mentioned

IPA
R
Gene Set Enrichment Analysis ( GSEA )
Ingenuity Pathway Analysis
ComplexHeatmap
GeneOverlap
DescTools
Ingenuity Pathway Analysis ( IPA )
R package
GSA

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