PMID: 19917734Nov 18, 2009Paper

Dynamic changes in matrix metalloproteinase 9 and tissue inhibitor of metalloproteinase 1 levels during wound healing in diabetic rats

Journal of the American Podiatric Medical Association
Chuan YangGuojuan Lao

Abstract

We investigated the mechanism of delayed would healing caused by diabetes and measured the dynamic changes in matrix metalloproteinase 9 (MMP-9) and tissue inhibitor of metalloproteinase 1 (TIMP-1) levels. We noted differences in the ratio of MMP-9 to TIMP-1 in the wounds of diabetic and nondiabetic rats. Forty-two Sprague-Dawley rats weighing 250 g were randomly assigned to either the control group or the streptozotocin-induced diabetes group. Then, full-thickness excision wounds were created on the middle of the back of each animal. Skin biopsy specimens were obtained on days 0, 3, 7, and 14 after incision. The content of collagen was quantified by Masson's staining and the macrophage marker, and CD68 was detected by immunohistochemical analysis. Messenger RNA and protein expression of MMP-9 and TIMP-1 was measured by reverse transcriptase-polymerase chain reaction and Western blot, respectively. Diabetic rats exhibited slower wound healing than control animals (P < .05). On days 3, 7, and 14 after incision, higher levels of MMP-9 messenger RNA and protein expression were detected in the diabetic group compared with the control group (P < .05), and expression of TIMP-1 messenger RNA and protein was significantly decreased. In...Continue Reading

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Citations

Apr 16, 2014·Journal of Diabetes and Its Complications·Jacqueline Chor-Wing TamClara Bik-San Lau
Apr 20, 2011·Journal of Cell Communication and Signaling·Christopher G Elliott, Douglas W Hamilton
May 12, 2012·Experimental Diabetes Research·Sheng-Neng XueLi Yan
Mar 2, 2012·The Journal of Investigative Dermatology·Sandra DannerJosé T Egaña
Apr 23, 2010·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Ursula MirastschijskiJames J Tomasek
Apr 26, 2011·The American Journal of Pathology·Dustin M BermudezKenneth W Liechty
Feb 26, 2016·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Jinglu ZhangLi Yan
Feb 28, 2016·Clinical Endocrinology·Qin TanMeng Ren
Jul 15, 2016·Journal of Wound Care·Y GuilleminJ N Gouze
Feb 19, 2013·Journal of Molecular Endocrinology·Li LingLi Yan
Dec 21, 2012·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Chrysanthos SymeonidisStavros A Dimitrakos
Jun 14, 2019·Journal of Periodontal Research·Meizi EliezerOfer Moses
May 29, 2012·Journal of Periodontology·Meizi EliezerOfer Moses
Apr 21, 2011·Biomedical Materials·Alistair S BrydoneLaura E McNamara
Jun 6, 2020·The International Journal of Lower Extremity Wounds·T S ShanmugarajanVaruna Naga Venkata Arjun Uppuluri
Apr 4, 2013·Critical Reviews in Biochemistry and Molecular Biology·Jennifer VandoorenGhislain Opdenakker
Mar 8, 2013·Journal of Cosmetic and Laser Therapy : Official Publication of the European Society for Laser Dermatology·Andreia Aparecida Da SilvaPaulo De Tarso Camillo De Carvalho
May 28, 2019·Pharmaceuticals·Jeffrey I JonesMayland Chang
Sep 13, 2014·World Journal of Emergency Medicine·Sheng-Neng XueLi Yan
Jun 23, 2021·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Rou WanRobert D Galiano
Jun 24, 2021·Journal of Cellular Physiology·Kehinde Ross

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