Dynamic changes in protein interaction between AKAP95 and Cx43 during cell cycle progression of A549 cells

Scientific Reports
Xiao-xuan ChenYong-xing Zhang

Abstract

Here we show that A-kinase anchoring protein 95 (AKAP95) and connexin 43 (Cx43) dynamically interact during cell cycle progression of lung cancer A549 cells. Interaction between AKAP95 and Cx43 at different cell cycle phases was examined by tandem mass spectrometry(MS/MS), confocal immunofluorescence microscopy, Western blot, and co-immunoprecipitation(Co-IP). Over the course of a complete cell cycle, interaction between AKAP95 and Cx43 occurred in two stages: binding stage from late G1 to metaphase, and separating stage from anaphase to late G1. The binding stage was further subdivided into complex binding to DNA in interphase and complex separating from DNA in metaphase. In late G1, Cx43 translocated to the nucleus via AKAP95; in anaphase, Cx43 separated from AKAP95 and aggregated between two daughter nuclei. In telophase, Cx43 aggregated at the membrane of the cleavage furrow. After mitosis, Cx43 was absent from the furrow membrane and was located in the cytoplasm. Binding between AKAP95 and Cx43 was reduced by N-(2-[P-Bromocinnamylamino]-ethyl)-5-isoquinolinesulfonmide (H89) treatment and enhanced by Forskolin. dynamic interaction between AKAP95 and Cx43 varies with cell cycle progression to regulate multiple biological pro...Continue Reading

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Citations

Dec 31, 2017·The FEBS Journal·Anna BieluszewskaElzbieta Poreba
May 9, 2019·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Xiaoming HouAamir Ahmed
Apr 19, 2017·Arkhiv patologii·E Yu KirichenkoP E Povilaitite
May 12, 2018·International Journal of Molecular Sciences·Paul L SorgenMorten S Nielsen
Feb 28, 2019·Analytical Cellular Pathology (Amsterdam)·Xiangyu KongYongxing Zhang
Mar 25, 2020·International Journal of Molecular Sciences·Saioa MendazaEsperanza Martín-Sánchez
Mar 16, 2021·Cellular Signalling·Angela L AsirvathamDavid J Carey
May 1, 2021·International Journal of Molecular Sciences·Antonio Rodríguez-SinovasIgnacio Ferreira-González
Nov 16, 2021·Molecular Medicine Reports·Hua-Chuan Zheng, Hua-Mao Jiang

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Datasets Mentioned

BETA
BGC823

Methods Mentioned

BETA
fluorescence imaging
flow cytometry
immunoprecipitation assay
Co-IP
Protein Assay
proximity ligation
confocal microscopy

Software Mentioned

SPSS21
Image Lab5

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