Dynamic changes in Sox2 spatio-temporal expression promote the second cell fate decision through Fgf4 /Fgfr2 signaling in preimplantation mouse embryos

The Biochemical Journal
Tapan Kumar MistriPaul Robson

Abstract

Oct4 and Sox2 regulate the expression of target genes such as Nanog, Fgf4, and Utf1, by binding to their respective regulatory motifs. Their functional cooperation is reflected in their ability to heterodimerize on adjacent cis regulatory motifs, the composite Sox/Oct motif. Given that Oct4 and Sox2 regulate many developmental genes, a quantitative analysis of their synergistic action on different Sox/Oct motifs would yield valuable insights into the mechanisms of early embryonic development. In the present study, we measured binding affinities of Oct4 and Sox2 to different Sox/Oct motifs using fluorescence correlation spectroscopy. We found that the synergistic binding interaction is driven mainly by the level of Sox2 in the case of the Fgf4 Sox/Oct motif. Taking into account Sox2 expression levels fluctuate more than Oct4, our finding provides an explanation on how Sox2 controls the segregation of the epiblast and primitive endoderm populations within the inner cell mass of the developing rodent blastocyst.

References

Jan 13, 1995·Science·B FeldmanM Goldfarb
Apr 25, 1994·Nucleic Acids Research·V R HarleyP N Goodfellow
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·E ArmanP Lonai
Jul 9, 2002·Current Opinion in Genetics & Development·Megan Wilson, Peter Koopman
Jan 7, 2003·Genes & Development·Ariel A AvilionRobin Lovell-Badge
Apr 30, 2005·The Journal of Biological Chemistry·David J RoddaPaul Robson
Aug 26, 2009·Cell·Jose SilvaAustin Smith
Sep 11, 2009·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Alexei A Sharov, Minoru S H Ko
Mar 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Samantha A MorrisMagdalena Zernicka-Goetz
Sep 23, 2011·Methods in Molecular Biology·William J R Longabaugh
Nov 15, 2011·Developmental Biology·Mila RoodeJennifer Nichols
Jun 25, 2013·Current Biology : CB·Yoshikazu HirateHiroshi Sasaki
May 20, 2014·Developmental Cell·Nadine SchrodeAnna-Katerina Hadjantonakis
Mar 11, 2015·Cell Reports·Panagiotis XenopoulosAnna-Katerina Hadjantonakis
Oct 16, 2015·Scientific Reports·Aleksandar I MihajlovićAlexander W Bruce

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Citations

Sep 27, 2019·Molecular Systems Biology·Daniel StrebingerDavid M Suter
Dec 6, 2019·Molecular Reproduction and Development·M Sofia OrtegaThomas E Spencer
May 10, 2020·The Analyst·Xuelin JinKyubong Jo
Oct 7, 2020·Annual Review of Cell and Developmental Biology·Claudia GerriKathy K Niakan
Feb 20, 2021·Nature Genetics·Žiga AvsecJulia Zeitlinger
Feb 15, 2021·Development, Growth & Differentiation·Yayoi Toyooka

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Methods Mentioned

BETA
transfection
fluorescence correlation spectroscopy
FCS
PCR
ChIP-Seq
transfections
FRET

Software Mentioned

BioTapestry
Biomark Real - time PCR Analysis
Image J
WebLogo
CisFinder
Fluidigm

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