PMID: 11906911Mar 22, 2002Paper

Dynamic changes in the expression of relaxin-like factor (INSL3), cholesterol side-chain cleavage cytochrome p450, and 3beta-hydroxysteroid dehydrogenase in bovine ovarian follicles during growth and atresia

Biology of Reproduction
Helen F Irving-RodgersRaymond J Rodgers

Abstract

Relaxin-like factor (RLF) is a new member of the insulin-relaxin gene family known to be expressed in the ovarian follicular thecal cells of ruminants. To investigate the pattern of RLF expression in development and atresia of bovine follicles, antisera were raised in rats and rabbits to recombinantly expressed bovine pro-RLF and to chemically synthesized ovine RLF B chain, respectively. On dot blotting analysis, the rat antiserum bound to pro-RLF and less strongly to a synthetic mature ovine RLF lacking the C-domain, whereas the rabbit antiserum bound the mature form of ovine RLF. These antisera were used to immunostain bovine ovarian follicles of differing sizes and stages of health and atresia. 3beta-Hydroxysteroid dehydrogenase was colocalized with pro-RLF (n = 86 follicles), and cholesterol side-chain cleavage cytochrome P450 was localized in another section of many of the same follicles (n = 66). Not all follicles expressed pro-RLF in the theca interna, so the results are presented as the proportion of follicles expressing pro-RLF. Both mature and pro-RLF were immunolocalized to steroidogenic thecal cells of healthy follicles. As follicles enlarged to >5 mm, the proportion expressing pro-RLF declined (19/19 for <5 mm and ...Continue Reading

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Citations

Dec 25, 2009·Cell and Tissue Research·Helen F Irving-RodgersRaymond J Rodgers
Jun 5, 2002·Molecular and Cellular Endocrinology·R J Rodgers, H F Irving Rodgers
Sep 10, 2002·Trends in Endocrinology and Metabolism : TEM·Richard Ivell, Almuth Einspanier
Mar 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Claire GlisterPhilip G Knight
Sep 10, 2013·Human Reproduction·R Anand-IvellR Ivell
Mar 31, 2009·Human Reproduction Update·Richard Ivell, Ravinder Anand-Ivell
Aug 24, 2002·Biology of Reproduction·Richard Ivell, Ross A D Bathgate
Jun 4, 2004·Biology of Reproduction·Leigh J ClarkRaymond J Rodgers
Feb 10, 2006·Biology of Reproduction·Ravinder J K Anand-IvellRichard Ivell
Nov 14, 2003·Reproductive Biology and Endocrinology : RB&E·Luiz E HenkesBo R Rueda
Feb 15, 2013·PloS One·Katja HummitzschRaymond J Rodgers
Jan 21, 2014·BMC Genomics·Nicholas HatzirodosRaymond J Rodgers
May 2, 2014·Journal of Molecular Endocrinology·Raifish E Mendoza-VillarroelJacques J Tremblay
Jan 23, 2013·Fertility and Sterility·Ravinder Anand-IvellRichard Ivell
Feb 9, 2010·Molecular and Cellular Endocrinology·Roy C K KongRoss A D Bathgate
Jun 16, 2005·Annals of the New York Academy of Sciences·Richard IvellRavinder Anand-Ivell
Jun 16, 2005·Annals of the New York Academy of Sciences·Nicole NicholsMichael J Fields
May 7, 2009·Annals of the New York Academy of Sciences·Jacques J TremblayEric Laguë
Aug 7, 2004·Matrix Biology : Journal of the International Society for Matrix Biology·Helen F Irving-RodgersRaymond J Rodgers
Jul 12, 2005·Molecular and Cellular Endocrinology·Helen SadeghianRichard Ivell
Sep 3, 2013·Molecular and Cellular Endocrinology·Ravinder Anand-Ivell, Richard Ivell
May 8, 2009·Biology of Reproduction·Ravinder Anand-IvellRichard Ivell
Jul 26, 2006·Molecular Reproduction and Development·H F Irving-RodgersR J Rodgers
Dec 7, 2018·Reproduction, Fertility, and Development·M D HartantiR J Rodgers
Mar 10, 2010·Cellular and Molecular Life Sciences : CMLS·G E Callander, R A D Bathgate
Mar 16, 2007·The Journal of Clinical Endocrinology and Metabolism·Alessandra GambineriRenato Pasquali
Jul 28, 2006·Molecular Human Reproduction·Helen F Irving-RodgersRaymond J Rodgers
Aug 25, 2009·Endocrinology·Richard Ivell, Frank Grutzner
Jan 11, 2013·Physiological Reviews·R A D BathgateR J Summers
Sep 12, 2018·Human Reproduction Update·Richard Ivell, Ravinder Anand-Ivell
Aug 18, 2020·General and Comparative Endocrinology·Richard IvellRavinder Anand-Ivell

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