Dynamic Changes in Yeast Phosphatase Families Allow for Specialization in Phosphate and Thiamine Starvation

G3 : Genes - Genomes - Genetics
John V NahasDennis D Wykoff

Abstract

Convergent evolution is often due to selective pressures generating a similar phenotype. We observe relatively recent duplications in a spectrum of Saccharomycetaceae yeast species resulting in multiple phosphatases that are regulated by different nutrient conditions - thiamine and phosphate starvation. This specialization is both transcriptional and at the level of phosphatase substrate specificity. In Candida glabrata, loss of the ancestral phosphatase family was compensated by the co-option of a different histidine phosphatase family with three paralogs. Using RNA-seq and functional assays, we identify one of these paralogs, CgPMU3, as a thiamine phosphatase. We further determine that the 81% identical paralog CgPMU2 does not encode thiamine phosphatase activity; however, both are capable of cleaving the phosphatase substrate, 1-napthyl-phosphate. We functionally demonstrate that members of this family evolved novel enzymatic functions for phosphate and thiamine starvation, and are regulated transcriptionally by either nutrient condition, and observe similar trends in other yeast species. This independent, parallel evolution involving two different families of histidine phosphatases suggests that there were likely similar se...Continue Reading

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Citations

Jul 17, 2020·Expert Review of Anti-infective Therapy·Mubashshir RasheedRupinder Kaur
Feb 2, 2019·Microorganisms·Kundan KumarRupinder Kaur
Aug 27, 2021·Journal of Fungi·Yahaya HassanLeslie Thian Lung Than

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Datasets Mentioned

BETA
CBS138
SRP131893

Methods Mentioned

BETA
RNA-seq
PCR
flow cytometry

Software Mentioned

Geospiza
HMMER
BLASTp
PhyML
Notung
BIONJ
BLAST
ProbCons

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