Dynamic changes of indoleamine 2,3-dioxygenase of Kupffer cells in rat liver transplant rejection and tolerance

Transplantation Proceedings
X LuanJ Li

Abstract

To study the dynamic changes and the immunologic role of indoleamine 2, 3-dioxygenase (IDO) in Kupffer cells (KCs) after rat liver transplantation. Animals were randomly divided into two groups: a rejection group (REJ; LEW to BN) and a tolerance group (TOL; BN to LEW). Liver morphological changes were observed optically with hematoxylin/eosin staining. KCs were isolated from recipients. mRNA and protein expressions of IDO were detected by real-time polymerase chain reaction and Western blotting at 1, 3, 5, and 7 days after transplantation. The levels of IDO mRNA and protein in KCs of TOL groups were similar to those in REJ groups at day 1 posttransplantation. However, the expression of IDO mRNA and protein time-dependently increased to much higher levels in the TOL than the in REJ groups at 3, 5, and 7 days posttransplantation (P < .05). The peak was observed at 7 days. The IDO level of KCs was closely associated with immune tolerance induction. IDO-mediated immune modulation appears to be an attractive means to assess transplant tolerance induction.

References

Mar 1, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases
Feb 1, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Vito Racanelli, Barbara Rehermann
Feb 8, 2006·Nature Medicine·Ken Shortman, Jose A Villadangos
May 24, 2007·Seminars in Liver Disease·Volker BenselerPatrick Bertolino
May 30, 2008·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Yong ChenJianping Gong
Feb 25, 2009·Seminars in Liver Disease·Stuart J Knechtle, Jean Kwun
Mar 24, 2009·Annual Review of Immunology·Ian Nicholas Crispe
Jul 1, 2009·Transplant Immunology·Lining JiaChenguang Ding
Apr 7, 2010·International Reviews of Immunology·Lei HuangAndrew L Mellor
Oct 26, 2010·Nature Reviews. Immunology·Angus W Thomson, Percy A Knolle

❮ Previous
Next ❯

Citations

Apr 5, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Hong ZhuFang Yang
Dec 10, 2013·Current Opinion in Organ Transplantation·Jessica H SpahnDaniel Kreisel

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

Autoimmune Hepatitis

Autoimmune hepatitis formerly called lupoid hepatitis, is a chronic, autoimmune disease of the liver that occurs when the body's immune system attacks liver cells causing the liver to be inflamed. Discover the latest research on autoimmune hepatitis here.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Related Papers

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
M Isabel Fiel, Thomas Schiano
American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons
Osamu YoshidaAngus W Thomson
Hepatology : Official Journal of the American Association for the Study of Liver Diseases
Frank A SchildbergPercy A Knolle
© 2021 Meta ULC. All rights reserved