PMID: 19143320Jan 16, 2009Paper

Dynamic changes of microbial community diversity in a photohydrogen producing reactor monitored by PCR-DGGE

Journal of Environmental Sciences (China)
Yanling YingJun Cheng

Abstract

A PCR-DGGE (denaturing gradient gel electrophoresis of polymerase chain reaction) protocol was used for monitoring the dynamic changes in the microbial population during photohydrogen production. Total DNA was extracted directly from the mixed bacterial community in the reactor and subjected to PCR with V3-16S rDNA and pufM gene primers, and the amplifications were then analyzed by DGGE. The DGGE patterns demonstrated the dynamics of community structure and the shift of microbial diversity, which corresponded to different running periods of the reactor. The optimal hydrogen producing community formed on day 10. Using DGGE analysis with the pufM gene fragments was superior to V3-16S rDNA region genes for detecting the dynamic variations of the photosynthetic bacteria population during hydrogen production. The comparative sequence analysis of excised DGGE bands showed the relationship between specific population structures and system performance. Rhodopseudomonas palustris was presumed as one of the dominant community members for hydrogen production in the reactor. The PCR-DGGE protocol was proven to be a good tool for monitoring the photohydrogen production in real time and offered the available information to improve the photoh...Continue Reading

References

Feb 13, 2001·Journal of Biotechnology·M J BarbosaR H Wijffels
Aug 18, 2004·Science·John A Turner
Jan 11, 2005·Applied and Environmental Microbiology·Aia OzOded Béjà
Oct 5, 2007·Journal of Environmental Sciences (China)·Qing HuHong-xun Zhang

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Citations

Jun 19, 2013·BMC Microbiology·Wenxiong LinRuiyu Lin
Oct 1, 2013·World Journal of Microbiology & Biotechnology·Changxun FangWenxiong Lin
Feb 5, 2014·Applied Microbiology and Biotechnology·Jingyin WangHongwei Rong
Apr 29, 2014·Bioresource Technology·Ahmed TawfikFaizal Bux
Apr 22, 2014·Bioresource Technology·Pradthana PrachanurakKazuo Yamamotob
Nov 12, 2019·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Zhi-Qing ZhaoAbbas Ghulam
Dec 15, 2010·Journal of Proteome Research·Hai-Bin WangWen-Xiong Lin

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