Dynamic changes of mitochondrial fusion and fission proteins after transient cerebral ischemia in mice

Journal of Neuroscience Research
Wentao LiuKoji Abe

Abstract

With fusion or fission, mitochondria alter their morphology in response to various physiological and pathological stimuli, resulting in elongated, tubular, interconnected, or fragmented forms. Immunohistochemistry and Western blot analysis were performed at 2 days, 7 days, 14 days, and 28 days after 90 min of transient middle cerebral artery occlusion (tMCAO) in mice. This study showed that mitochondrial fission protein dynamin-related protein 1 (Drp1) and fusion protein optic atrophy 1 (Opa1) were both upregulated in the ischemic penumbra, with the peak at 2 days after tMCAO, whereas phosphorylated-Drp1 (P-Drp1) progressively increased with a peak at 14 days after tMCAO. Double-immunofluorescence analysis showed many Drp1/cytochrome c oxidase subunit l (COX1) double-positive cells and Opa1/COX1 double-positive cells in the ischemic penumbra and also showed some double-positive cells with Drp1/terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end labeling (TUNEL) and Opa1/TUNEL in the ischemic penumbra. In contrast, both Drp1 and Opa1 showed progressive decreases until 2 days after tMCAO in the ischemic core because of necrotic brain damage. The present study suggests that there was a continuous mitochondrial...Continue Reading

References

Jan 1, 1977·Stroke; a Journal of Cerebral Circulation·J AstrupN A Lassen
Aug 1, 1995·Stroke; a Journal of Cerebral Circulation·K AbeY Itoyama
Feb 15, 1994·Microscopy Research and Technique·J Bereiter-Hahn, M Vöth
Sep 1, 1993·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·K AbeK Kogure
Jul 1, 1993·Brain Research. Molecular Brain Research·K AbeK Kogure
Jun 5, 2003·The Journal of Biological Chemistry·Dominic I JamesJean-Claude Martinou
Nov 14, 2003·Nature·Katrin Henze, William Martin
Oct 2, 2004·The Journal of Biological Chemistry·Rie SugiokaYoshihide Tsujimoto
Feb 25, 2005·Neurological Research·Takeshi Hayashi, Koji Abe
Nov 16, 2005·Annual Review of Genetics·Koji Okamoto, Janet M Shaw
Feb 10, 2006·The New England Journal of Medicine·Kennedy R LeesUNKNOWN Stroke-Acute Ischemic NXY Treatment (SAINT I) Trial Investigators
May 18, 2006·Annual Review of Cell and Developmental Biology·David C Chan
Mar 3, 2007·FEBS Letters·Yan Zhang, David C Chan
Mar 17, 2007·Annual Review of Biochemistry·Suzanne HoppinsJodi Nunnari
Jun 1, 2007·The Journal of Biological Chemistry·Arezu Jahani-AslRuth S Slack
Jun 8, 2007·The Journal of Biological Chemistry·Chuang-Rung Chang, Craig Blackstone
Sep 14, 2007·Clinical Pharmacology and Therapeutics·D J Hausenloy, L Scorrano
Oct 12, 2007·Nature Reviews. Molecular Cell Biology·Scott A Detmer, David C Chan
Apr 4, 2008·Trends in Pharmacological Sciences·Elaine BesanconEng H Lo
Jun 24, 2008·Nature Reviews. Neuroscience·Andrew B KnottElla Bossy-Wetzel
Aug 13, 2008·The Journal of Cell Biology·Xiao-Jian HanMasayuki Matsushita
Oct 8, 2008·Proceedings of the National Academy of Sciences of the United States of America·G M CereghettiL Scorrano
May 30, 2009·Molecular Biology of the Cell·Zhiyin SongDavid C Chan
Oct 6, 2009·Biochimica Et Biophysica Acta·Bo SuXiongwei Zhu
Oct 8, 2009·Human Molecular Genetics·Hsiuchen Chen, David C Chan
Oct 12, 2010·Autophagy·FengFeng TianKoji Abe

❮ Previous
Next ❯

Citations

Mar 19, 2014·International Journal of Molecular Sciences·Li ZhangJie Jia
May 2, 2013·Oxidative Medicine and Cellular Longevity·Zhanyang YuXiaoying Wang
Apr 9, 2013·Neurochemical Research·Kangyong LiuXiaojiang Sun
Oct 25, 2012·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·R Anne StetlerJun Chen
Jun 22, 2013·Journal of Neurochemistry·Vasco SilveirinhaHelena Cimarosti
Mar 21, 2015·Biochemical and Biophysical Research Communications·Shang-Der ChenYao-Chung Chuang
Sep 25, 2014·Journal of Bioenergetics and Biomembranes·Katrina OwensTibor Kristian
Apr 30, 2014·Journal of Stroke and Cerebrovascular Diseases : the Official Journal of National Stroke Association·Jinying WangMin Wang
Sep 30, 2016·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Justin BalogRaghu Vemuganti
Jul 1, 2017·Therapeutic Hypothermia and Temperature Management·Jong Youl KimMidori A Yenari
Sep 25, 2017·Translational Stroke Research·Nina KlimovaTibor Kristian
Jul 9, 2016·Stroke; a Journal of Cerebral Circulation·Jong Youl KimMidori A Yenari
Dec 6, 2018·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Carlos Roberto Porto DechandtLuciane Carla Alberici
Jan 18, 2017·Wiley Interdisciplinary Reviews. RNA·Donald J DeGracia
Jan 23, 2020·Stem Cells and Development·Lei HuangJohn H Zhang
Feb 20, 2019·Frontiers in Neuroscience·Xiu-Yun ZhaoQuan-Hong Ma
Oct 3, 2018·Aging and Disease·Fan LiuQin Hu
Feb 26, 2019·Journal of Neuroscience Research·Nina KlimovaTibor Kristian
Jun 2, 2020·Translational Neurodegeneration·Luodan YangQuanguang Zhang
Nov 10, 2020·Free Radical Biology & Medicine·Armita Mahdavi GorabiAmirhossein Sahebkar

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.