Dynamic compartmentalization of protein tyrosine phosphatase receptor Q at the proximal end of stereocilia: implication of myosin VI-based transport

Cell Motility and the Cytoskeleton
Hirofumi SakaguchiBechara Kachar

Abstract

Hair cell stereocilia are apical membrane protrusions filled with uniformly polarized actin filament bundles. Protein tyrosine phosphatase receptor Q (PTPRQ), a membrane protein with extracellular fibronectin repeats has been shown to localize at the stereocilia base and the apical hair cell surface, and to be essential for stereocilia integrity. We analyzed the distribution of PTPRQ and a possible mechanism for its compartmentalization. Using immunofluorescence we demonstrate that PTPRQ is compartmentalized at the stereocilia base with a decaying gradient from base to apex. This distribution can be explained by a model of transport directed toward the stereocilia base, which counteracts diffusion of the molecules. By mathematical analysis, we show that this counter transport is consistent with the minus end-directed movement of myosin VI along the stereocilia actin filaments. Myosin VI is localized at the stereocilia base, and exogenously expressed myosin VI and PTPRQ colocalize in the perinuclear endosomes in COS-7 cells. In myosin VI-deficient mice, PTPRQ is distributed along the entire stereocilia. PTPRQ-deficient mice show a pattern of stereocilia disruption that is similar to that reported in myosin VI-deficient mice, whe...Continue Reading

References

Jun 16, 1997·The Journal of Cell Biology·T HassonD P Corey
Oct 20, 1999·Developmental Biology·T SelfK P Steel
Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·B KacharP G Gillespie
Nov 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·R S RockH L Sweeney
Aug 23, 2002·Nature·Mark E SchneiderBechara Kachar
Jun 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·A OganesianD F Bowen-Pope
Jul 15, 2003·Molecular Biology of the Cell·Laura AschenbrennerTama Hasson
Oct 4, 2003·Physical Review Letters·Ilan TsafrirJoel Stavans
Dec 20, 2003·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Thorsten Auth, Gerhard Gompper
Mar 17, 2004·The Journal of Cell Biology·Agnieszka K RzadzinskaBechara Kachar
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Folma BussJohn Kendrick-Jones
Jan 14, 2005·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Rhys RobertsJohn Kendrick-Jones
Oct 11, 2005·Biophysical Journal·Erdinç AtilganSean X Sun
Feb 17, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Mathias SenftenUlrich Müller
Mar 31, 2006·Molecular Biology of the Cell·Tatsuhiko NoguchiKathryn G Miller
Jun 10, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·M'hamed GratiBechara Kachar
Jun 16, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Joann McGeeGuy P Richardson
Apr 13, 2007·Annual Review of Neuroscience·Melissa A VollrathDavid P Corey
Jun 15, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Nicolas MichalskiChristine Petit

❮ Previous
Next ❯

Citations

Jan 29, 2010·Cellular and Molecular Life Sciences : CMLS·Rajalakshmi NambiarMatthew J Tyska
Nov 3, 2011·Nature Communications·Anthony W PengAnthony J Ricci
Jan 22, 2013·Nature Neuroscience·Jung-Bum ShinPeter G Barr-Gillespie
Sep 12, 2009·Human Molecular Genetics·Isabelle RouxSaaid Safieddine
Aug 1, 2013·Human Molecular Genetics·Rafael PulidoWiljan J A J Hendriks
Feb 24, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Richard J GoodyearGuy P Richardson
Mar 30, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Hongyu ZhaoPeter G Gillespie
May 2, 2014·European Journal of Human Genetics : EJHG·Celia Zazo SecoMargit Schraders
Jul 14, 2010·The Journal of Cell Biology·Martin SchwanderUlrich Müller
Dec 28, 2011·Hearing Research·Meghan C DrummondThomas B Friedman
Aug 18, 2010·Developmental Neurobiology·Gowri NayakGuy P Richardson
Oct 21, 2010·Biophysical Journal·Martin LenzJean-François Joanny
Aug 8, 2014·Biophysical Journal·G OrlyN S Gov
Aug 2, 2014·Developmental Neurobiology·Naveen Prakash Bokolia, Monalisa Mishra
Feb 6, 2015·Human Molecular Genetics·Misato YoshikawaJin-ichi Inokuchi
Oct 5, 2014·International Journal of Pediatric Otorhinolaryngology·Hossein Najmabadi, Kimia Kahrizi
Jun 25, 2014·Pflügers Archiv : European journal of physiology·Nicolas Michalski, Christine Petit
Mar 20, 2015·The Annals of Otology, Rhinology, and Laryngology·Naoko SakumaShin-Ichi Usami
Mar 31, 2015·The Annals of Otology, Rhinology, and Laryngology·Shin-Ya NishioShin-Ichi Usami
Aug 16, 2016·ELife·Clive P MorganPeter G Barr-Gillespie
Apr 26, 2012·Experimental Animals·Yoshiaki KikkawaHiromichi Yonekawa
Jan 9, 2019·Cold Spring Harbor Perspectives in Medicine·Guy P Richardson, Christine Petit
Jul 8, 2020·Journal of Medical Genetics·Suzanne E de BruijnUNKNOWN DOOFNL Consortium
Oct 28, 2019·Journal of Translational Medicine·Dominika OziębłoMonika Ołdak
Sep 2, 2016·The Journal of Biological Chemistry·Manmeet H RavalChristopher M Yengo
Mar 13, 2014·Journal of Cell Science·Takehiko UeyamaNaoaki Saito
May 26, 2021·Current Biology : CB·Anne Houdusse, Margaret A Titus
Sep 17, 2009·Current Opinion in Otolaryngology & Head and Neck Surgery

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.