Dynamic compartmentation of vacuolar amino acids in Penicillium cyclopium. Cytosolic adenylates act as a control signal for efflux into the cytosol.

The Journal of Biological Chemistry
W RoosJ Steighardt

Abstract

The regulation of amino acid transport from the vacuolar reservoir into the cytoplasm has been studied in hyphal cells of Penicillium cyclopium. To avoid artifacts caused by the isolation of vacuoles, efflux was examined "in situ," i.e. in cells whose plasma membranes were permeabilized for micromolecules by a treatment with nystatin. The ATP-dependent proton gradient and amino acid transport activities at the vacuolar membrane remained intact under these conditions. Accumulation of amino acids in the vacuole proved to be the result of a dynamic equilibrium of active, ATP-dependent uptake and energy-independent efflux. The latter was strongly accelerated after the vacuolar amino acid content had surpassed a threshold level. Efflux of vacuolar amino acids was specifically controlled by extravacuolar adenylates: ATP, 5'-adenylyl imidodiphosphate (an ATPase-resistant ATP-analogue), ADP, or AMP caused a strong inhibition in the concentration range around 200 micromol/liter, whereas both lower and higher concentrations allowed significant efflux rates. Estimates of the cytosolic adenylates (which consisted mainly of ATP) were close to 2 mmol/liter in glucose-metabolizing cells, which concentration allowed maximum rates of both vacuo...Continue Reading

References

May 1, 1975·European Journal of Biochemistry·T BollerA Wiemken
Jan 1, 1978·Journal of Supramolecular Structure·R H DavisR L Weiss
Mar 11, 1975·Journal of Bacteriology·W J Ball, D E Atkinson
Aug 1, 1992·Journal of Bioenergetics and Biomembranes·H SzeS Lai
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A Bertl, C L Slayman
Oct 1, 1989·Journal of Bioenergetics and Biomembranes·Y AnrakuY Wada
Dec 22, 1986·Biochimica Et Biophysica Acta·J Horák
May 1, 1982·Journal of Bacteriology·C Drainas, R L Weiss
May 1, 1982·Journal of Bacteriology·C Drainas, R L Weiss
Apr 29, 1994·Biochemical and Biophysical Research Communications·R ColomboL Giromini

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Citations

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May 18, 1999·Fungal Genetics and Biology : FG & B·M P BrownG A Payne
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Mar 27, 1999·The Journal of Biological Chemistry·C FörsterJ L Revuelta
Apr 18, 1998·The Journal of Biological Chemistry·R YoshidaO Yoshie
Dec 9, 2000·The Journal of Biological Chemistry·P LoetscherI Clark-Lewis
Jan 29, 2003·The Journal of Biological Chemistry·Mark E DeVriesDavid J Kelvin
Jan 27, 1998·The Journal of Biological Chemistry·T ImaiP W Gray
Jan 23, 2004·The Journal of Biological Chemistry·Julio GutiérrezGabriel Márquez
Feb 7, 1998·The Journal of Biological Chemistry·R HorukR W Doms
Feb 7, 1998·The Journal of Biological Chemistry·M Wong, E N Fish

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