Dynamic contributions to the DNA binding entropy of the EcoRI and EcoRV restriction endonucleases

Journal of Molecular Biology
Y DuanJ M Rosenberg

Abstract

Molecular Dynamics simulations on DNA-EcoRI and DNA-EcoRV complexes suggest that the DNA within these complexes is significantly more ordered than free DNA. Similarly, both the protein and the DNA are more ordered in the specific (cognate) DNA-EcoRV complex than they are in the non-cognate DNA-protein complex, consistent with recently proposed analogies between protein folding and sequence-specific DNA-protein recognition. Analysis of the trajectories shows that the net entropy gain upon specific binding to be the result of opposing contributions. Solvent release, which increases entropy versus configurational terms (as measured by the magnitude of the atomic fluctuations), and collective terms from tight coupling between the motions of the protein and the DNA.

Citations

Nov 24, 1999·Journal of Biomolecular Structure & Dynamics·R H WingerE Mayer
Jun 20, 2006·Journal of Biomolecular Structure & Dynamics·Pemra DorukerOzge Kurkcuoglu
Feb 17, 2001·Journal of Molecular Recognition : JMR·A P Demchenko

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