Dynamic coupling of ALCAM to the actin cortex strengthens cell adhesion to CD6

Journal of Cell Science
Joost Te RietDaniel J Müller

Abstract

At the immunological synapse, the activated leukocyte cell adhesion molecule (ALCAM) on a dendritic cell (DC) and CD6 molecules on a T cell contribute to sustained DC-T-cell contacts. However, little is known about how ALCAM-CD6 bonds resist and adapt to mechanical stress. Here, we combine single-cell force spectroscopy (SCFS) with total-internal reflection fluorescence microscopy to examine ALCAM-CD6-mediated cell adhesion. The combination of cells expressing ALCAM constructs with certain cytoplasmic tail mutations and improved SCFS analysis processes reveal that the affinity of ALCAM-CD6 bonds is not influenced by the linking of the intracellular domains of ALCAM to the actin cortex. By contrast, the recruitment of ALCAM to adhesion sites and the propensity of ALCAM to anchor plasma membrane tethers depend on actin cytoskeletal interactions. Furthermore, linking ALCAM to the actin cortex through adaptor proteins stiffens the cortex and strengthens cell adhesion. We propose a framework for how ALCAMs contribute to DC-T-cell adhesion, stabilize DC-T-cell contacts and form a mechanical link between CD6 and the actin cortex to strengthen cell adhesion at the immunological synapse.

References

Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·J J GrootjansG David
Feb 7, 2001·Science·F OesterheltD J Müller
Apr 18, 2001·The Journal of Biological Chemistry·L C van KempenG W Swart
May 2, 2001·Nature Reviews. Molecular Cell Biology·M P Sheetz
Oct 2, 2003·Current Opinion in Cell Biology·Christopher V Carman, Timothy A Springer
Jan 9, 2004·Nature·Thorsten R MempelUlrich H Von Andrian
Mar 30, 2004·European Journal of Immunology·Namir J HassanMarion H Brown
Aug 6, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Idoia GimferrerFrancisco Lozano
May 18, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Benjamin CrossJean-Paul Rieu
Jul 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Idoia GimferrerFrancisco Lozano
Aug 20, 2005·Cellular Signalling·Christer Larsson
Sep 27, 2005·Biophysical Journal·Mingzhai SunMichel Grandbois
May 26, 2007·Science·Evan A Evans, David A Calderwood
Oct 10, 2007·Current Opinion in Cell Biology·Michael L Dustin
Nov 10, 2007·Proceedings of the National Academy of Sciences of the United States of America·Veronika I ZarnitsynaCheng Zhu
Apr 25, 2008·Journal of Cell Science·Jeffrey M Beekman, Paul J Coffer
May 22, 2008·Journal of Cell Science·Jonne HeleniusDaniel J Muller
Feb 11, 2009·The Journal of Cell Biology·Jian-Jiang HaoStephen Shaw
Oct 24, 2009·Proceedings of the National Academy of Sciences of the United States of America·Thomas S van ZantenMaria F Garcia-Parajo
Mar 24, 2010·Nature Reviews. Molecular Cell Biology·Richard G FehonAnthony Bretscher
Nov 19, 2011·Ultramicroscopy·Joost te RietSylvia Speller
Feb 22, 2012·Journal of Cell Science·Mónica Sala-ValdésMaría Yáñez-Mó
Jul 6, 2012·Nature·Prithu SunddKlaus Ley
Jul 17, 2012·Journal of Cell Science·Ulrich S Schwarz, Margaret L Gardel
Nov 3, 2012·Journal of Biomechanics·Igor TitushkinMichael Cho
Nov 20, 2012·Frontiers in Physiology·Zhongkui HongGabor Forgacs
Oct 4, 2013·Journal of Molecular Recognition : JMR·Lu DaoClemens M Franz

❮ Previous
Next ❯

Citations

Aug 26, 2015·The Journal of Cell Biology·Amanda Carroll-PortilloDiane S Lidke
Jul 7, 2015·Structure·Paul E ChappellMarion H Brown
Feb 12, 2015·Beilstein Journal of Nanotechnology·Miao YuJonne Helenius
Jan 10, 2020·Molecular Microbiology·Felipe VielaYves F Dufrêne
Mar 26, 2014·The Journal of Biological Chemistry·Cicerone TudorVinod Subramaniam
Apr 9, 2020·Frontiers in Immunology·Leila AbdelhamidXin M Luo
Nov 26, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anoek van RijnSonja I Buschow
Jan 11, 2017·Proceedings of the National Academy of Sciences of the United States of America·Marc-André LécuyerAlexandre Prat
Jul 14, 2018·Cellular & Molecular Immunology·Anja Meyer, David M Kofler
May 16, 2018·Frontiers in Immunology·Raquel ReyesCarlos Cabañas

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Cell Adhesion Molecules in the Brain

Cell adhesion molecules found on cell surface help cells bind with other cells or the extracellular matrix to maintain structure and function. Here is the latest research on their role in the brain.