Abstract
The present study investigated the dynamic expression and regulatory mechanism of transforming growth factor β (TGF-β) signaling involved in embryonic stem cells (ESCs) differentiation into male germ cells. Candidate genes involved in TGF-β signaling pathway were screened from RNA-sequencing (RNA-seq), which were further validated by quantitative real-time PCR (qRT-PCR). Bone morphogenetic protein 4 (BMP4) was used to induce differentiation of ESCs in vitro Inhibition of TGF-β signaling pathway was reflected by Western blot of SMAD2 and SMAD5 expression. Differentiating efficiency of germ cells was evaluated by immunofluorescence and fluorescence-activated cell sorting (FACS). Germ cell marker genes were assessed by qRT-PCR in the differentiation process, with activation or inhibition of TGF-β signaling pathway. In the process of in vitro induction, SMAD2 and SMAD5 were found to significantly up-regulated in BMP4 group versus the control and inhibition groups after 4 and 14 days. Expression of CKIT, CVH, DAZL, STRA8, and INTEGRIN α6 were significantly increased in the BMP4 group compared with the control group, while down-regulated in the inhibition groups. The proportion of germ cell-like cells was decreased from 17.9% to 2.2%...Continue Reading
References
Nov 15, 2001·Pharmacology & Therapeutics·J Yue, K M Mulder
Sep 25, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yayoi ToyookaToshiaki Noce
Dec 12, 2003·Nature·Niels GeijsenGeorge Q Daley
Dec 23, 2003·Cellular Signalling·Anja NoheNils O Petersen
Feb 11, 2004·Endocrine Reviews·Shunichi ShimasakiGregory F Erickson
Feb 20, 2004·Development·Eihachiro KawaseTing Xie
Nov 9, 2004·Developmental Biology·Jie HuGuang-Quan Zhao
Nov 23, 2006·Mechanisms of Development·Brian M DudleyKathleen Molyneaux
Jul 1, 2008·Annual Review of Cell and Developmental Biology·Jon M Oatley, Ralph L Brinster
Sep 16, 2008·Molecular and Cellular Endocrinology·Mushtaq A MemonMichael K Skinner
Mar 6, 2009·Microscopy Research and Technique·Zuping HeMartin Dym
May 5, 2009·Cell·Yasuhide OhinataMitinori Saitou
Oct 21, 2009·Human Reproduction·F D WestS L Stice
Mar 30, 2010·Developmental Biology·Stéphanie G MorenoRené Habert
Mar 11, 2011·Biology of Reproduction·Stephanie C BannisterMark L V Tizard
Apr 28, 2011·In Vitro Cellular & Developmental Biology. Animal·Zohreh MakoolatiMehdi Forouzandeh-Moghadam
Sep 6, 2011·Acta histochemica·Xue-Jing ZhangJun-Ping He
Feb 2, 2012·Biochemical and Biophysical Research Communications·Wen ChenJiuhong Kang
May 3, 2014·Animal Reproduction Science·Qing-Qing ShiBi-Chun Li
Apr 8, 2015·The Journal of Biological Chemistry·Zhentao ZhangBichun Li
Citations
Sep 13, 2017·Journal of Cellular Biochemistry·Man WangBichun Li
May 14, 2019·Journal of Cellular Biochemistry·Xiaoliang YangYinghao Li
Mar 10, 2020·Cellular Reprogramming·Chunxia XiongXiong Xiao
Mar 15, 2019·Stem Cell Research & Therapy·Yanxia LuoMeijin Guo
Jun 29, 2018·Cell & Bioscience·Qisheng ZuoBichun Li
Jan 15, 2021·Journal of Cell Science·Qisheng ZuoBichun Li
Mar 28, 2021·Cell Death & Disease·Wangyue JiaQiong Wu