Dynamic expression of ganglion cell markers in retinal progenitors during the terminal cell cycle.

Molecular and Cellular Neurosciences
Lev Prasov, Tom Glaser

Abstract

The vertebrate neural retina contains seven major cell types, which arise from a common multipotent progenitor pool. During neurogenesis, these cells stop cycling, commit to a single fate, and differentiate. The mechanism and order of these steps remain unclear. The first-born type of retinal neurons, ganglion cells (RGCs), develop through the actions of Math5 (Atoh7), Brn3b (Pou4f2) and Islet1 (Isl1) factors, whereas inhibitory amacrine and horizontal precursors require Ptf1a for differentiation. We have examined the link between these markers, and the timing of their expression during the terminal cell cycle, by nucleoside pulse-chase analysis in the mouse retina. We show that G2 phase lasts 1-2 h at embryonic (E) 13.5 and E15.5 stages. Surprisingly, we found that cells expressing Brn3b and/or Isl1 were frequently co-labeled with EdU after a short chase (<1 h) in early embryos (<E14), indicating that these factors, which mark committed RGCs, can be expressed during S or G2 phases. However, during late development (>E15), Brn3b and Isl1 were exclusively expressed in post-mitotic cells, even as new RGCs are still generated. In contrast, Ptf1a and amacrine marker AP2α were detected only after terminal mitosis, at all development...Continue Reading

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Citations

Mar 17, 2015·Proceedings of the National Academy of Sciences of the United States of America·Fuguo WuXiuqian Mu
Dec 18, 2013·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Marek Pacal, Rod Bremner
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Jun 15, 2019·International Journal of Molecular Sciences·Adam M MiltnerAnna La Torre
Mar 5, 2017·The EMBO Journal·Peter EngererLeanne Godinho
Jan 9, 2019·Frontiers in Genetics·Ying LiEldon E Geisert
Aug 21, 2020·Proceedings of the National Academy of Sciences of the United States of America·Joel B MiesfeldTom Glaser
Jul 1, 2018·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Paula Quintero-Ronderos, Paul Laissue
Oct 4, 2020·BMC Evolutionary Biology·R G LoosemoreT C Stanger
Jan 27, 2015·Development·Manuel StemmerJoachim Wittbrodt

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