Dynamic fatigue measurement of human erythrocytes using dielectrophoresis

Acta Biomaterialia
Yuhao QiangE Du

Abstract

Erythrocytes must undergo severe deformation to pass through narrow capillaries and submicronic splenic slits for several hundred thousand times in their normal lifespan. Studies of erythrocyte biomechanics have been mainly focused on cell deformability and rheology measured from a single application of stress and mostly under a static or quasi-static state using classical biomechanical techniques, such as optical tweezers and micropipette aspiration. Dynamic behavior of erythrocytes in response to cyclic stresses that contributes to the membrane failure in blood circulation is not fully understood. This paper presents a new experimental method for dynamic fatigue analysis of erythrocytes, using amplitude modulated electrokinetic force field in a microfluidic platform. We demonstrate the capability of this new technique using a low cycle fatigue analysis of normal human erythrocytes and ATP-depleted erythrocytes. Cyclic tensile stresses are generated to induce repeated uniaxial stretching and extensional recovery of single erythrocytes. Results of morphological and biomechanical parameters of individually tracked erythrocytes show strong correlations with the number of the loading cycles. Under a same strength of electric field...Continue Reading

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Citations

Jun 1, 2018·Journal of Biomedical Optics·Michele KelleyDaniel B Erenso
Sep 19, 2019·Proceedings of the National Academy of Sciences of the United States of America·Yuhao QiangE Du
Dec 5, 2019·Biomedical Optics Express·Mehrzad SasanpourS Nader S Reihani
Apr 24, 2018·Applied Sciences·E DuJia Liu
Apr 20, 2021·ACS Biomaterials Science & Engineering·Elyahb A KwizeraXiaoming He
Jul 8, 2021·Biotechnology and Bioengineering·Darryl DieujusteE Du

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