Dynamic Generation of Concentration- and Temporal-Dependent Chemical Signals in an Integrated Microfluidic Device for Single-Cell Analysis

Analytical Chemistry
Alan M Gonzalez-SuarezJose L Garcia-Cordero


Intracellular signaling pathways are affected by the temporal nature of external chemical signaling molecules such as neurotransmitters or hormones. Developing high-throughput technologies to mimic these time-varying chemical signals and to analyze the response of single cells would deepen our understanding of signaling networks. In this work, we introduce a microfluidic platform to stimulate hundreds of single cells with chemical waveforms of tunable frequency and amplitude. Our device produces a linear gradient of 9 concentrations that are delivered to an equal number of chambers, each containing 492 microwells, where individual cells are captured. The device can alternate between the different stimuli concentrations and a control buffer, with a maximum operating frequency of 33 mHz that can be adjusted from a computer. Fluorescent time-lapse microscopy enables to obtain hundreds of thousands of data points from one experiment. We characterized the gradient performance and stability by staining hundreds of cells with calcein AM. We also assessed the capacity of our device to introduce periodic chemical stimuli of different amplitudes and frequencies. To demonstrate our device performance, we studied the dynamics of intracellu...Continue Reading


Jul 3, 2003·Nature Reviews. Molecular Cell Biology·Michael J BerridgeH Llewelyn Roderick
Jun 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jessica OlofssonOwe Orwar
Jun 15, 2007·International Review of Cytology·Geneviève DupontLuc Leybaert
Jun 28, 2007·Lab on a Chip·L ChenC H Mastrangelo
Mar 19, 2009·Molecular Systems Biology·Naotoshi NakamuraMasamitsu Iino
May 30, 2009·Analytical Chemistry·Alar AinlaAldo Jesorka
Jul 30, 2009·Science Signaling·William S Hlavacek, James R Faeder
Oct 20, 2010·Current Opinion in Genetics & Development·Marcelo Behar, Alexander Hoffmann
May 12, 2011·Molecular BioSystems·Andreja JovicShuichi Takayama
Oct 19, 2011·Current Opinion in Genetics & Development·Alexander Loewer, Galit Lahav
Mar 5, 2013·Cell·Jeremy E Purvis, Galit Lahav
Jun 5, 2013·Integrative Biology : Quantitative Biosciences From Nano to Macro·Andreja JovicShuichi Takayama
Nov 22, 2016·Integrative Biology : Quantitative Biosciences From Nano to Macro·M SumitJ J Linderman
Feb 7, 2017·Integrative Biology : Quantitative Biosciences From Nano to Macro·Ariel S Kniss-JamesMelissa L Kemp
Apr 14, 2017·Analytical Chemistry·Rocio J Jimenez-ValdesJose L Garcia-Cordero
Jun 3, 2017·Lab on a Chip·Andreas PiehlerSavaş Tay
Aug 10, 2017·Analytical Chemistry·Peng ChenBi-Feng Liu
Dec 1, 2017·Analytical Chemistry·Kristina Woodruff, Sebastian J Maerkl
Feb 8, 2018·Trends in Cell Biology·Carlotta GiorgiPaolo Pinton
Apr 19, 2018·Lab on a Chip·Po-Hsun HuangTony Jun Huang

❮ Previous
Next ❯


Aug 11, 2020·Clinical and Experimental Otorhinolaryngology·Se Hwan HwangAlexander Revzin
Mar 6, 2019·Analytical Chemistry·Diana F Cedillo-AlcantarAlexander Revzin
May 11, 2019·ACS Sensors·Xiaoyan LiuXingyu Jiang
Jan 14, 2020·ACS Sensors·Roberto Rodriguez-MoncayoJose Luis Garcia-Cordero
Oct 27, 2018·Analytical Chemistry·Pieter E OomenAndrew G Ewing

❮ Previous
Next ❯

Related Concepts

Related Feeds

Advanced Imaging of Cellular Signaling

Cell signaling is a vital mechanism for communication within cells and outside with the environment. Several different signaling pathways have been found and advanced imaging techniques are being developed to visualize the molecules involved in these signaling pathways. Find the latest research in advanced imaging of cellular signaling here.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.