Dynamic Interaction of Stress Granules, DDX3X, and IKK-α Mediates Multiple Functions in Hepatitis C Virus Infection

Journal of Virology
Véronique PèneT Jake Liang

Abstract

The ubiquitous ATP-dependent RNA helicase DDX3X is involved in many cellular functions, including innate immunity, and is a pivotal host factor for hepatitis C virus (HCV) infection. Recently, we showed that DDX3X specifically recognizes the HCV 3' untranslated region (UTR), leading to the activation of IKK-α and a cascade of lipogenic signaling to facilitate lipid droplet biogenesis and viral assembly (Q. Li, V. Pene, S. Krishnamurthy, H. Cha, and T. J. Liang, Nat Med 19:722-729, 2013, http://dx.doi.org/10.1038/nm.3190). The interaction of DDX3X with HCV core protein seems to be dispensable for its proviral role. In this study, through systematic imaging and biochemical and virologic approaches, we identified a dynamic association between DDX3X and various cellular compartments and viral elements mediating multiple functions of DDX3X in productive HCV infection. Upon HCV infection, the HCV 3'UTR interacts with DDX3X and IKK-α, which redistribute to speckle-like cytoplasmic structures shown to be stress granules (SGs). As viral proteins accumulate in infected cells, DDX3X granules together with SG-associated proteins redistribute and colocalize with HCV core protein around lipid droplets (LDs). IKK-α, however, does not relocate...Continue Reading

References

May 21, 1999·The Journal of Biological Chemistry·N Mamiya, H J Worman
Dec 22, 1999·Journal of Viral Hepatitis·C U Hellen, T V Pestova
Mar 19, 2003·The Journal of Cell Biology·Helene TourrièreJamal Tazi
Mar 3, 2004·Nature Reviews. Molecular Cell Biology·Sanda Rocak, Patrick Linder
Sep 17, 2004·Molecular Biology of the Cell·Natalie GilksPaul Anderson
Apr 14, 2005·Journal of Hepatology·Karim AbidFrancesco Negro
Jan 28, 2006·Journal of Virology·Jennifer A SmithLeslie A Schiff
Feb 16, 2007·Journal of Virology·Takanobu KatoT Jake Liang
Jul 10, 2007·Proceedings of the National Academy of Sciences of the United States of America·Glenn RandallCharles M Rice
Aug 28, 2007·Nature Cell Biology·Yusuke MiyanariKunitada Shimotohno
Sep 15, 2007·Journal of Virology·Yasuo AriumiNobuyuki Kato
Nov 17, 2007·Cell Host & Microbe·James P WhiteRichard E Lloyd
Feb 23, 2008·Trends in Biochemical Sciences·Paul Anderson, Nancy Kedersha
Apr 15, 2008·Cell Host & Microbe·Carla J Beckham, Roy Parker
Jul 17, 2008·Nucleic Acids Research·Chung-Sheng LeeRobin Reed
Mar 7, 2009·The Journal of General Virology·Mair HughesMark Harris
Sep 1, 2009·Proceedings of the National Academy of Sciences of the United States of America·Qisheng LiStephen J Elledge
Jan 22, 2010·Journal of Virology·Yong-Yuan ZhangT Jake Liang
Feb 4, 2010·European Journal of Immunology·Hiroyuki OshiumiTsukasa Seya
Sep 17, 2010·Journal of Virology·Michael E LindquistJames E Crowe
Oct 1, 2010·The Journal of General Virology·G MousseauA D Strosberg
Mar 25, 2011·Biochemical Society Transactions·Martina Schröder
May 6, 2011·Journal of Virology·Yasuo AriumiNobuyuki Kato
Dec 7, 2011·FEMS Microbiology Reviews·Robert ThimmeRalf Bartenschlager
Feb 11, 2012·Nucleic Acids Research·Rene GeisslerSven-Erik Behrens
Mar 13, 2012·Trends in Microbiology·James P White, Richard E Lloyd
Oct 16, 2012·Journal of Hepatology·Markus H Heim
May 17, 2013·The New England Journal of Medicine·T Jake Liang, Marc G Ghany
Jun 15, 2013·Nucleic Acids Research·Chaomin SunJamie H D Cate

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Citations

Jan 10, 2016·Journal of Biochemistry·Mitsutoshi YoneyamaKoji Onomoto
Jul 2, 2016·Viruses·Natalia Poblete-DuránFernando Valiente-Echeverría
Sep 10, 2016·Frontiers in Microbiology·Ana L C ValadãoLuciana B de Arruda
Feb 2, 2018·Frontiers in Cellular and Infection Microbiology·Rinki KumarGuruprasad R Medigeshi
Feb 6, 2017·Journal of Virology·Bita KhadivjamRoger Lippé
Oct 21, 2018·The Biochemical Journal·Anthony FullamMartina Schröder
May 1, 2019·Virologica Sinica·Qiang ZhangMingzhou Chen
Feb 28, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marina K KukhanovaAlexander V Ivanov
Jul 16, 2015·Reviews in Medical Virology·Fernando Valiente-EcheverríaRicardo Soto-Rifo
Aug 7, 2017·Liver International : Official Journal of the International Association for the Study of the Liver·Carlos Fernández-CarrilloSofía Pérez-Del-Pulgar
Nov 19, 2019·Frontiers in Microbiology·Gayatri D KanadeYogesh A Karpe
Mar 6, 2018·Frontiers in Microbiology·Saba Valadkhan, Puri Fortes
Oct 17, 2017·Frontiers in Microbiology·Marina Barriocanal, Puri Fortes
Nov 5, 2019·Frontiers in Cellular and Infection Microbiology·Aracelly Gaete-ArgelFernando Valiente-Echeverría
Sep 10, 2020·Viruses·Nina EiermannAlessia Ruggieri
Feb 25, 2017·Virus Genes·Viviana FalcónJuan Kourı
May 11, 2018·Biotechnology & Genetic Engineering Reviews·Vanessa Meier-StephensonTrushar R Patel
Mar 28, 2020·International Journal of Molecular Sciences·Juan P Unfried, P Fortes
Jan 20, 2021·Cellular & Molecular Immunology·Koji OnomotoMitsutoshi Yoneyama
Oct 30, 2020·International Journal of Molecular Sciences·Alexandra P M ClohertyFrançois Jean
Apr 30, 2021·Science Advances·Qi LiXiaoming Zhang

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