Dynamic interactions of c-fos protein in serum-stimulated 3T3 cells

Journal of Cellular Physiology
R J VosatkaE B Ziff

Abstract

The c-fos gene, the cellular homologue of the transforming gene of the FBJ osteosarcoma virus, v-fos, is strongly induced in quiescent BALB/c 3T3 cells by growth factors and in other cell types by a wide variety of transmembrane signalling agents. c-fos is a member of a family of structurally related proteins which includes the fos-related antigens (fra). We have studied the dynamic state of the c-fos protein with an antibody prepared by immunizing rabbits with a plasmid-encoded fos fusion protein. In serum-stimulated BALB/c 3T3 cells, the antibody recognizes a nuclear antigen which resolves on SDS-PAGE as a 60-68-kD group of bands corresponding to c-fos, a doublet at 44-45-kD corresponding to the noncovalently associated p39 protein, as well as an approximately 50-kD band corresponding to a fra. We show that although c-fos protein synthesis is only transiently induced by serum, the c-fos protein persists within the cell after its synthesis has ceased, and it decays with a half-life of 2 hours. Significantly, newly synthesized p39 continues to appear in the immune-precipitated complex even at times when c-fos is no longer synthesized. These kinetics indicate that even following shutoff of c-fos protein synthesis, p39 is newly s...Continue Reading

References

Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A M Maxam, W Gilbert
Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·W J PledgerC D Scher
Apr 1, 1988·Molecular and Cellular Biology·K T RiabowolJ R Feramisco
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·W KruijerI M Verma
May 20, 1988·Science·F J RauscherB R Franza
May 1, 1988·Molecular and Cellular Biology·D R Cohen, T Curran
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J T HoltA W Nienhuis
Feb 1, 1987·Molecular and Cellular Biology·K Nishikura, J M Murray
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P K VogtR F Doolittle
Jan 1, 1985·Molecular and Cellular Biology·T CurranI M Verma

❮ Previous
Next ❯

Citations

Dec 15, 1990·Brain Research. Developmental Brain Research·J D GorhamE B Ziff
Sep 1, 1995·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·H WangH S Schwartz

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.