Dynamic Inventory of Intermediate Metabolites of Cyanobacteria in a Diurnal Cycle

IScience
Damini Jaiswal, Pramod P Wangikar

Abstract

Cyanobacteria are gaining importance both as hosts for photoautotrophic production of chemicals and as model systems for studies of diurnal lifestyle. The proteome and transcriptome of cyanobacteria have been closely examined under diurnal growth, whereas the downstream effects on the intermediary metabolism have not received sufficient attention. The present study focuses on identifying the cellular metabolites whose inventories undergo dramatic changes in a fast-growing cyanobacterium, Synechococcus elongatus PCC 11801. We identified and quantified 67 polar metabolites, whose inventory changes significantly during diurnal growth, with some metabolites changing by 100-fold. The Calvin-Benson-Bassham cycle intermediates peak at midday to support fast growth. The hitherto unexplored γ-glutamyl peptides act as reservoirs of amino acids. Interestingly, several storage molecules or their precursors accumulate during the dark phase, dispelling the notion that all biosynthetic activity takes place in the light phase. Our results will guide metabolic modeling and strain engineering of cyanobacteria.

References

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Citations

Oct 12, 2021·Bioresource Technology·Ruchi PathaniaPratyoosh Shukla
Oct 26, 2021·Current Opinion in Biotechnology·Damini JaiswalPramod P Wangikar
Oct 22, 2021·Frontiers in Plant Science·Nathaphon Yu King HingJohn A Morgan
Nov 3, 2021·The Plant Journal : for Cell and Molecular Biology·Damini JaiswalPramod P Wangikar

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Methods Mentioned

BETA
genetic modifications
glycosylation
reverse-phase chromatography

Software Mentioned

DIAL
X 13 CMS
MetDIA
MS

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