Dynamic Labeling Reveals Temporal Changes in Carbon Re-Allocation within the Central Metabolism of Developing Apple Fruit

Frontiers in Plant Science
Wasiye F BeshirBart M Nicolaï

Abstract

In recent years, the application of isotopically labeled substrates has received extensive attention in plant physiology. Measuring the propagation of the label through metabolic networks may provide information on carbon allocation in sink fruit during fruit development. In this research, gas chromatography coupled to mass spectrometry based metabolite profiling was used to characterize the changing metabolic pool sizes in developing apple fruit at five growth stages (30, 58, 93, 121, and 149 days after full bloom) using (13)C-isotope feeding experiments on hypanthium tissue discs. Following the feeding of [U-(13)C]glucose, the (13)C-label was incorporated into the various metabolites to different degrees depending on incubation time, metabolic pathway activity, and growth stage. Evidence is presented that early in fruit development the utilization of the imported sugars was faster than in later developmental stages, likely to supply the energy and carbon skeletons required for cell division and fruit growth. The declined (13)C-incorporation into various metabolites during growth and maturation can be associated with the reduced metabolic activity, as mirrored by the respiratory rate. Moreover, the concentration of fructose an...Continue Reading

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BETA
genetic modification
PCA

Software Mentioned

Unscrambler® X
Agilent MSD Chemstation Software
MS Correction Tool
MultiExperiment Viewer
JMP

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