Dynamic light scattering analysis of SNARE-driven membrane fusion and the effects of SNARE-binding flavonoids

Biochemical and Biophysical Research Communications
Yoo-Soo YangDae-Hyuk Kweon

Abstract

Soluble N-ethylmaleimide-sensitive-factor attachment protein receptor (SNARE) proteins generate energy required for membrane fusion. They form a parallelly aligned four-helix bundle called the SNARE complex, whose formation is initiated from the N terminus and proceeds toward the membrane-proximal C terminus. Previously, we have shown that this zippering-like process can be controlled by several flavonoids that bind to the intermediate structures formed during the SNARE zippering. Here, our aim was to test whether the fluorescence resonance energy transfer signals that are observed during the inner leaflet mixing assay indeed represent the hemifused vesicles. We show that changes in vesicle size accompanying the merging of bilayers is a good measure of progression of the membrane fusion. Two merging vesicles with the same size D in diameter exhibited their hydrodynamic diameters 2D + d (d, intermembrane distance), 2D and 2D as membrane fusion progressed from vesicle docking to hemifusion and full fusion, respectively. A dynamic light scattering assay of membrane fusion suggested that myricetin stopped membrane fusion at the hemifusion state, whereas delphinidin and cyanidin prevented the docking of the vesicles. These results a...Continue Reading

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Citations

Nov 3, 2016·Proceedings of the National Academy of Sciences of the United States of America·Arti B PatelTheoharis C Theoharides
Apr 14, 2017·The Journal of Pharmacology and Experimental Therapeutics·Arti B Patel, Theoharis C Theoharides
Apr 15, 2017·Frontiers in Molecular Neuroscience·Paul HeoDae-Hyuk Kweon
Aug 6, 2020·Scientific Reports·Choongjin BanDae-Hyuk Kweon
Apr 23, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Choongjin BanDae-Hyuk Kweon
Feb 19, 2021·European Biophysics Journal : EBJ·Hannes WittAndreas Janshoff
Apr 8, 2021·Journal of Peptide Science : an Official Publication of the European Peptide Society·Barbara E HubrichUlf Diederichsen
Sep 27, 2019·Journal of Agricultural and Food Chemistry·Zhixi ChenLonghuo Wu

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