Dynamic Loading and Tendon Healing Affect Multiscale Tendon Properties and ECM Stress Transmission

Scientific Reports
Benjamin R FreedmanLouis J Soslowsky

Abstract

The extracellular matrix (ECM) is the primary biomechanical environment that interacts with tendon cells (tenocytes). Stresses applied via muscle contraction during skeletal movement transfer across structural hierarchies to the tenocyte nucleus in native uninjured tendons. Alterations to ECM structural and mechanical properties due to mechanical loading and tissue healing may affect this multiscale strain transfer and stress transmission through the ECM. This study explores the interface between dynamic loading and tendon healing across multiple length scales using living tendon explants. Results show that macroscale mechanical and structural properties are inferior following high magnitude dynamic loading (fatigue) in uninjured living tendon and that these effects propagate to the microscale. Although similar macroscale mechanical effects of dynamic loading are present in healing tendon compared to uninjured tendon, the microscale properties differed greatly during early healing. Regression analysis identified several variables (collagen and nuclear disorganization, cellularity, and F-actin) that directly predict nuclear deformation under loading. Finite element modeling predicted deficits in ECM stress transmission following...Continue Reading

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Citations

Mar 26, 2019·Advanced Materials·Benjamin R Freedman, David J Mooney
Feb 6, 2019·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Cody D HillinLouis J Soslowsky
Jul 10, 2019·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Andrey ZuskovLouis J Soslowsky
Jan 15, 2020·Connective Tissue Research·Stefania L WunderliJess G Snedeker
Feb 23, 2020·Nature Reviews. Rheumatology·Eric GraceyDirk Elewaut
Apr 26, 2020·International Journal of Molecular Sciences·Mersedeh TohidnezhadThomas Pufe
Nov 3, 2020·Soft Matter·Farid AlisafaeiVivek B Shenoy
Feb 16, 2021·Current Tissue Microenvironment Reports·Connor C LeekMegan L Killian
May 26, 2021·Connective Tissue Research·Monideepa ChatterjeeNelly Andarawis-Puri
Jun 3, 2021·International Journal of Molecular Sciences·Iris RibitschFlorien Jenner
Jun 22, 2021·Frontiers in Cell and Developmental Biology·Yuwei YangHongwei Ouyang
Jul 30, 2021·Acta Biomaterialia·Patrick M WilliamsonAra Nazarian
Oct 7, 2021·Science Translational Medicine·Bo Ri SeoDavid J Mooney
Dec 23, 2021·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Benjamin R FreedmanLouis J Soslowsky
Jan 9, 2022·Biomechanics and Modeling in Mechanobiology·Tianyi RenHannah L Dailey

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Methods Mentioned

BETA
biopsy
dissection

Software Mentioned

ImageJ
SPSS
MATLAB
CellProfiler
FIJI

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