Dynamic Loading and Unloading of Proteins in Polymeric Stomatocytes: Formation of an Enzyme-Loaded Supramolecular Nanomotor.

ACS Nano
L K E A AbdelmohsenDaniela A Wilson

Abstract

Self-powered artificial nanomotors are currently attracting increased interest as mimics of biological motors but also as potential components of nanomachinery, robotics, and sensing devices. We have recently described the controlled shape transformation of polymersomes into bowl-shaped stomatocytes and the assembly of platinum-driven nanomotors. However, the platinum encapsulation inside the structures was low; only 50% of the structures contained the catalyst and required both high fuel concentrations for the propelling of the nanomotors and harsh conditions for the shape transformation. Application of the nanomotors in a biological setting requires the nanomotors to be efficiently propelled by a naturally available energy source and at biological relevant concentrations. Here we report a strategy for enzyme entrapment and nanomotor assembly via controlled and reversible folding of polymersomes into stomatocytes under mild conditions, allowing the encapsulation of the proteins inside the stomach with almost 100% efficiency and retention of activity. The resulting enzyme-driven nanomotors are capable of propelling these structures at low fuel concentrations (hydrogen peroxide or glucose) via a one-enzyme or two-enzyme system. ...Continue Reading

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Citations

May 25, 2016·ACS Nano·Jinxing LiJoseph Wang
Jun 28, 2016·Small·David A GregoryStephen J Ebbens
Jul 5, 2016·Journal of the American Chemical Society·Loai K E A AbdelmohsenJan C M van Hest
Sep 27, 2016·ACS Nano·Xing MaSamuel Sánchez
Oct 21, 2016·Nanoscale·Fernando SotoJoseph Wang
Nov 5, 2016·Accounts of Chemical Research·Jaideep KaturiSamuel Sánchez
Jan 25, 2017·Journal of the American Chemical Society·Renfeng DongYuepeng Cai
Feb 12, 2017·ACS Nano·Yingfeng TuDaniela A Wilson
Mar 23, 2017·ACS Nano·Philipp S SchattlingBrigitte Städler
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Apr 22, 2017·Nature Chemistry·Yingfeng TuDaniela A Wilson
Aug 14, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Motilal MatheshDaniela A Wilson

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